DOI: https://doi.org/10.22263/2312-4156.2023.3.38
N.Ye. Maksimovich, L.I. Bon, E.M. Doroshenko, V.Yu. Smirnov, Yu.Ye. Razvodovsky, D.G. Khilkevich, A.M. Portonenko
Abnormalities of the brain amino acid pool in rats with partial cerebral ischemia
Grodno State Medical University, Grodna, Republic of Belarus
Vestnik VGMU. 2023;22(3):38-44.
Abstract.
Cerebrovascular diseases are widespread both in the Republic of Belarus and throughout the world. In these diseases, the pool of amino acids in the brain largely changes.
Objectives. To study changes in the pool of amino acids (AA) in the brain of rats with partial cerebral ischemia (PCI).
Material and methods. The experiments were performed on 16 male outbred white rats weighing 260±20 g. Cerebral ischemia was modelled under conditions of intravenous thiopental anesthesia (40–50 mg/kg). PCI was modelled by ligation of one common carotid artery on the right. The material was taken 1 hour after the operation.
Results. One-hour PCI is characterized by the following changes in the AA pool: an increase in glutamate and gamma aminobutyric acid (GABA) without the changes in the ratio of excitatory and inhibitory amino acids-transmitters, a decrease in the level of essential AAs with an increase in the “Essential/Non-essential” ratio, a decrease in the level of aromatic AAs (tyrosine, tryptophan, phenylalanine) and those with a branched hydrocarbon chain. There were no changes in sulfur-containing AAs (except for methionine in the parietal lobe), which indicates slight violations of the prooxidant-oxidant balance.
Conclusions. Changes in the pool of amino acids in the parietal lobe and hippocampus were of the same nature, with the exception of a more pronounced decrease in the level of methionine in the parietal lobe as a reflection of a higher activity of oxidative processes in this area of the brain.
Keywords: ischemia, amino acid pool, rat, brain.
Source of financing. State Scientific and Technical Program, subprogram “Translational Medicine”.
References
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Information about authors:
N.Ye. Maksimovich – Doctor of Medical Sciences, professor, head of the Chair of Pathological Physiology named after D.A. Maslakov, Grodno State Medical University, https://orcid.org/0000-0003-3181-9513;
L.I. Bon – Candidate of Biological Sciences, associate professor of the Chair of Pathological Physiology named after D.A. Maslakov, Grodno State Medical University, https://orcid.org/0000-0001-7189-0838;
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Lizaveta I. Bon;
E.M. Doroshenko – Candidate of Biological Sciences, associate professor of the Chair of Biological Chemistry, Grodno State Medical University, https://orcid.org/0000-0001-9939-8749;
V.Yu. Smirnov – Candidate of Biological Sciences, senior researcher of the research laboratory, Grodno State Medical University;
Yu.Ye. Razvodovsky – Candidate of Biological Sciences, research officer of the Institute of Biochemistry, Belarusian National Academy of Sciences;
D.G. Khilkevich – the third-year pediatric student, Grodno State Medical University;
A.M. Portonenko – the third-year pediatric student, Grodno State Medical University.
