DOI: https://doi.org/10.22263/2312-4156.2023.3.86
A.T. Shchastniy, A.S. Osochuk, S.S. Osochuk, A.F. Martsinkevich
Changes of fatty acids spectrum of very low density lipoproteins in patients with chronic renal failure and in the early terms after kidney transplantation
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2023;22(3):86-96.
Abstract.
Objectives. To evaluate the fatty acid spectrum of native very low density lipoproteins (VLDL) in patients with terminal chronic renal failure and in the early terms after kidney transplantation.
Material and methods. The study group included 15 men (36-60 years old) and 15 women (36-55 years old). The control group included 15 healthy men and 15 healthy women of the same age. Patients’ blood was taken before kidney transplantation, before the first administration of tacrolimus, in 24 hours and on day 7 after kidney transplantation. VLDL were isolated by preparative ultracentrifugation method. The spectrum of fatty acids was determined by gas chromatography. Statistical analysis was performed using the application package R version 4.0.5 (2021-03-31).
Results. A statistically significant decrease in the content of the following fatty acids was revealed in patients with CRF: myristic (C14:0), myristoleic (C14:1), pentadecanoic (C15:0), pentadecenoic (C15:1), palmitic (C16 :0), trans-isomer of linoleic acid (C18:2n6t), arachidic (C20:0), eicosadienoic (C20:2), erucic (C22:1), docosadienoic (C22:2) and lignoceric (C24:0). In addition, an increase in the content of oleic (C18:1n9c), linoleic (C18:2n6c), arachidonic acid (C20:4n6) in patients of the same group was revealed. The evaluation of the spectrum of fatty acids in patients with end-stage renal failure after transplantation of a donor kidney showed almost identical changes, indicating the persistence of the imbalance of fatty acids. However, there were some differences: an increase in the content of palmitic acid (C16:0) after 24 hours and on day 7 after the operation, a decrease in the content of C18:1n9t and ℽ-linolenic acid (C18:3n6c). In addition, a decrease in the ratio of saturated and monounsaturated fatty acids and an increase in the ratio of unsaturated and polyunsaturated fatty acids were found.
Conclusions. Changes in the fatty acid spectrum of native VLDL were mainly of a negative proatherogenic nature, probably associated, among other things, with a disturbance of the production of fatty acids by the intestinal microbiota, contributing to the growth of insulin resistance.
Keywords: VLDL, fatty acids, tacrolimus, insulin resistance, kidney transplantation, CRF.
Information about the source of support in the form of grants, equipment, medicinal agents. The research was carried out within the frames of the State Research Program (GPNI) “Translational medicine”, subprogram 4.2 “Fundamental aspects of medical science”, task 3.37 “To study the condition of lipid transport and immune systems of patients with kidney transplantation and to substantiate the approaches to their correction”, State Registration No. 20220305 dated 16.03.2022.
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Information about authors:
A.T. Shchastniy – Doctor of Medical Sciences, professor, head of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, ORCID: https://orcid.org/0000-0003-2796-4240
A.S. Osochuk – postgraduate of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-5942-3601
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Alexander S. Osochuk;
S.S. Osochuk – Doctor of Medical Sciences, professor, head of the research laboratory, Vitebsk State Order of Peoples’ Friendship Medical University, ORCID: https://orcid.org/0000-0003-2074-3832
A.F. Martsinkevich – Candidate of Biological Sciences, associate professor of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0003-3655-4489
