DOI: https://doi.org/10.22263/2312-4156.2023.4.97
O.A. Yakovleva1, R.I. Lukashov2, L.A. Lyubakovskaya1, S.V. Tsaprilova3
Variability of the phenolic composition of buds and leaves of Syringa vulgaris L. root growth during the main phases of vegetation of a mother plant
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Belarusian State Medical University, Minsk, Republic of Belarus
3Vitebsk TPRUЕ “Pharmacy”, Vitebsk, Republic of Belarus
Vestnik VGMU. 2023;22(4):97-104.
Abstract.
Objectives. To establish the composition and to analyze the specific content of phenolic compounds in the buds and leaves of Syringa vulgaris L. root growth during the main vegetation phases of a mother plant.
Material and methods. The object of the study was the buds and leaves of the root shoots of wild Syringa vulgaris L., collected during the main 6 phases of the plant’s vegetation, determined according to the method of phenological observations, growing in the north part of the city of Vitebsk. The composition was determined by high-performance liquid chromatography (HPLC).
Results. The component composition and specific content of phenolic compounds of the buds and leaves of the root growth of Syringa vulgaris L. is unstable and depends on the phenological phases of plant development. The maximum content of isoquercitrin – 76.9% is observed in the budding phase of the mother plant, the maximum accumulation of m-n-rutinoside – 8%, unidentified flavonoid 1 – 22.7% and unidentified flavonoid 2 – 13.1% can be detected in the lignification phase of the mother plant, the content of oleuropein –31.8% and of unidentified flavonoid 3 –21.8% reaches its maximum in the phase of fruit ripening on the mother plant.
Conclusions. The buds and leaves of root shoots can be recommended as a promising source for obtaining such substances as oleuropein, isoquercitrin and m-n-rutinoside. These substances have cardioprotective, anti-inflammatory, antioxidant, antiangiogenic, neuroprotective and antitumor effects. Conducting further studies to establish the structure of unidentified compounds is relevant due to their high content in the buds and leaves of the root shoots of Syringa vulgaris L.
Keywords: leaves and root shoots of common lilac, HPLC, Syringa vulgaris, isoquercitrin, m-n-rutinoside, oleuropein.
References
1. Polyakova NV. Seasonal rhythm of development of Syringavulgaris L. species in the city of Ufa. Vestn IrGSKhA. 2011;(44-2):120-5. (In Russ.)
2. Tsapalova IE, Golub OV, Gubina MD, Deryusheva TV, Poznyakovskiy VM; Poznyakovskiy VM, red. Examination of wild fruits, berries and herbaceous plants. Quality and safety: uchebnik. 6-e izd, pererab i dop. Moskva, RF: INFRA-M; 2018. 463 р. (In Russ.)
3. Lomboeva SS, Tankhaeva LM, Olennikov DN. Dynamics of flavonoid accumulation in the above-ground part of lopsided ortilia (Orthilia secunda (L.) house). Khimiya Rastit Syr'ya. 2008;(3):83-8. (In Russ.)
4. Mirovich VM, Fedoseeva GM, Bocharova GI, Chepoguzova AV. Dynamics of flavonoids and tannins content in aboveground organs of Rhododendron dauricum. Byul VSNTs SO RAMN. 2005;(6):149-51. (In Russ.)
5. Manyakhin AYu, Kolbin PS. Dynamics of flavonoid content in raw materials of Baikal hellebore. Tikhookean Med Zhurn. 2013;(2):60-1. (In Russ.)
6. Yakovlev GP, red. Pharmacognosy. Medicinal raw materials of plant and animal origin: ucheb posobie. St. Petersburg, RF: SpetsLit; 2013. 847 р. (In Russ.)
7. TKP 450-2012 (02041), BY. Manufacture of medicinal products. Good practice of cultivation, collection, storage of medicinal plant raw materials = Vytvorchasts' lekavykh srodkaў. Nalezhnaya praktyka vyroshchvannya, zboru, zakhoўvannya lekavai raslіnnai syravіny. Vved 01.03.13. Minsk, RB: Departament farmatsevt prom-sti; 2013. 14 р. (In Russ.)
8. Korozhan NV, Buzuk GN. Comparative analysis of the component composition of alcoholic extracts from the herb of species of succession by liquid chromatography method. Vestn Farmatsii. 2013;(4):49-56. (In Russ.)
9. M-vo zdravookhraneniya Resp Belarus', UP «Tsentr ekspertiz i ispytanii v zdravookhranenii»; Sheryakov AA, red. State Pharmacopoeia of the Republic of Belarus: in 2 vol. T 1: General methods of quality control of medicinal products. Molodechno, RB: Pobeda; 2012. 1220 р. (In Russ.)
Information about authors:
O.A. Yakovleva – Candidate of Biological Sciences, head of the Chair of Standardization of Medicines with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Olga A. Yakovleva;
R.I. Lukashov – Candidate of Pharmaceutical Sciences, associate professor, head of the Chair of Organization of Pharmacy, Belarusian State Medical University;
L.A. Lyubakovskaya – Candidate of Biological Sciences, associate professor of the Chair of Biology and Pharmaceutical Botany, Vitebsk State Order of Peoples’ Friendship Medical University;
S.V. Tsaprilova – head of pharmacy No. 320 of Vitebsk TPRUE “Pharmacy”.
