DOI: https://doi.org/10.22263/2312-4156.2023.5.18
V.Y. Ziamko
The influence of diallyl trisulfide and copper nanoparticles on the morphology and functional activity of the liver
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2023;22(5):18-25.
Abstract.
Objectives. To study the effect of diallyl trisulfide and copper nanoparticles on the liver.
Material and methods. There were 3 groups of white male rats weighing 250-300 g: group 1 (n=7) was the control group, it was not exposed to any influences, group 2 (n=5) took diallyl trisulfide intragastrically daily, group 3 (n=5) got copper nanoparticles intramuscularly every 7 days. Blood and liver samples were taken for biochemical, histological and immunohistochemical studies.
Results. There was 1.2 times reliable decrease of total protein in both experimental groups (р=0.005). Aspartate aminotransferase was 1.8 times higher in diallyl trisulfide group and made up 244 [232.9-264.5] U/L (р=0.022). Total bilirubin was 1.8 times higher in group 3 and made up 26.5; [26.2-27.9] µmol/l (р=0.005). Intragastric administration of diallyl trisulfide and copper nanoparticles increased compensatory adaptive reactions of the liver due to 2.3 times increase in the number of binuclear hepatocytes in both experimental groups (p=0.009; p=0.001, respectively). The percentage of CD68 expression in healthy group was 62%, in copper nanoparticles group – 55.2% (p=0.082), in diallyl trisulfide group – 53.1% (p=0.080) and didn’t differ.
Conclusions. The administration of diallyl trisulfide and copper nanoparticles reduced the level of total protein in blood serum, increased serum levels of aspartate aminotransferase in diallyl trisulfide group and total bilirubin in copper nanoparticles group, which indicates the presence of liver damage and loss of its function. In response to introduction of diallyl trisulfide and copper nanoparticles 2.3 times increase in the binuclear hepatocytes number was observed. It indicated an increase of compensatory adaptive reactions of the liver.
Keywords: diallyl trisulfide, copper nanoparticles, liver, hepatotoxicity, compensatory adaptive reactions, CD68.
The work has been done within the frames of the research theme “The influence of liver dysfunction on the development of systemic inflammatory response”, the agreement with BRFFR No. M23M-046 dated 02.05.2023.
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Information about authors:
V.Y. Ziamko – Candidate of Medical Sciences, associate professor of the Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-6753-2074,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viktoriya Y. Ziamko.
