DOI: https://doi.org/10.22263/2312-4156.2023.5.61
A.A. Marchenko, V.M. Semenov, S.K. Egorov, T.I. Dmitrachenko, I.A. Lyatos
The detection of Torque teno virus DNA in the lacrimal fluid of patients with cataract and concomitant ophthalmopathology
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2023;22(5):61-66.
Abstract.
Torque teno virus (TT-virus, TTV) is often found in various tissues of the human body. This virus is widely spread in the population. The role of this virus in the human virome is currently being studied, due to the evidence of lifelong persistence of TTV and a variety of interactions with the human body, including immune cells. Various studies demonstrate the presence of TTV in the eye tissues of patients with different ocular pathologies, which indicates an association between TT virus and eye diseases. Therefore, the determination of the frequency of TTV persistence in the tissues and fluids of the eye is of interest.
The aim of the study was to determine the frequency of Torque teno virus occurrence in the lacrimal fluid in patients with cataract. The study included 60 patients (33 men and 27 women). The samples of stimulated tear fluid were taken from the conjunctival cavity. The detection of TTV DNA was carried out using real-time polymerase chain reaction.
TTV DNA was detected in 23 out of 60 (38.5%) tear fluid samples. The frequent detection of TTV in the tear fluid confirms its role in the ocular virome and formulates the hypothesis that the tear may be a route of transmission of TT infection. It may also indicate that the presence of TTV in the aqueous humor and vitreous body may be a risk factor for the development of various eye diseases. These results give rise to interest in further research in this direction.
Keywords: TTV, cataract, lacrimal fluid, polymerase chain reaction, virome.
The research was carried out within the frames of the State Research Program (GPNI) “To detect the levels of TTV DNA, reflecting the intensity of cellular immune response in bacteremia caused by Klebsiella Pneumoniae, Acinetobacter Baumanii, Pseudomonas aeruginosa”, State Registration No. 20220336.
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Information about authors:
A.A. Marchenko – lecturer of the Chair of Ophthalmology, Vitebsk State Order of Peoples’ Friendship Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Arina A. Marchenko;
V.M. Semenov – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
S.K. Egorov – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
T.I. Dmitrachenko – Doctor of Medical Sciences, professor, dean of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
I.A. Lyatos – Candidate of Medical Sciences, associate professor, head of the Chair of Military Training & Emergency Medicine, Vitebsk State Order of Peoples’ Friendship Medical University.
