DOI: https://doi.org/10.22263/2312-4156.2023.5.73
O.G. Sechko1, O.A. Stepuro1, N.S. Golyak1, Z.N. Bragina1, V.M. Tsarenkov1, E.N. Kalinichenko2, T.S. Bozhok2
Behavioral reactions and morphological characteristic of inner organs of rats while determining subacute toxicity of 3-[4-(2-fluorobenzoyl) piperazine-1-carbonyl]-N-[(3-(trifluoromethyl) phenyl] benzamide
1Belarusian State Medical University, Minsk, Republic of Belarus
2Institute of Bioоrganic Chemistry of the National Academy of Sciences of Belarus, Minsk, Republic of Belarus
Vestnik VGMU. 2023;22(5):73-81.
Abstract.
The aim of this work is to study the motor and the orientation-exploratory activity of rats in the “open field” test as well as the morphological changes in the internal organs of rats to determine the subacute toxicity of the benzamide derivative – 3-[4-(2-fluorobenzoyl)piperazine-1-carbonyl]-N-[3-(trifluoromethyl) phenyl] benzamide, which possesses an antitubercular activity. In the “open field” test performed on Wistar rats on the 28th day of the experiment after daily intragastric administration of an aqueous suspension of the benzamide derivative at doses of 10 mg/kg, 50 mg/kg and 100 mg/kg, there were no statistically significant differences in motor and orientation-exploratory activity of rats of the experimental groups in comparison with the control group (p>0.05). Visible changes in the internal organs of rats in the experimental groups and in the control group were not found. Histological examination of the morphological features of the internal organs of rats in the control group, group 1 (10 mg/kg), group 2 (50 mg/kg) and group 3 (100 mg/kg) did not reveal any pathological changes, some individual morphological characteristics were found that did not go beyond the features usually observed in intact rats, which were taken into account in the histological examination of the internal organs of rats. The results of the conducted study indicate that 3-[4-(2-fluorobenzoyl)piperazine-1-carbonyl]-N-[3-(trifluoromethyl)phenyl] benzamide is a non-toxic compound in a subacute experiment on Wistar rats.
Keywords: benzamide derivative, 3-[4-(2-fluorobenzoyl)piperazine-1-carbonyl]-N-[3-(trifluoromethyl)phenyl]benzamide, subacute toxicity, “open field” test, histological examination of internal organs of rats.
The research was carried out within the frames of the State Research Program (GPNI) “Chemical processes, reagents and technologies, bioregulators and bioorganic chemistry”, task “Obtaining, assessment of antimicrobial activity and safety of some derivatives of benzamide and benzoic acid” (State Registration No. 20230397, 2023-2025).
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Information about authors:
O.G. Sechko – Master of Pharmaceutical Sciences, senior lecturer of the Chair of Pharmaceutical Technology, Belarusian State Medical University, https://orcid.org/0000-0001-5727-9936
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Olga G. Sechko;
O.A. Stepuro – senior lecturer of the Chair of Pathological Anatomy, Belarusian State Medical University;
N.S. Golyak – Candidate of Pharmaceutical Sciences, associate professor, head of the Chair of Pharmaceutical Technology, Belarusian State Medical University, https://orcid.org/0000-0002-4904-6523.
Z.N. Bragina – Candidate of Medical Sciences, associate professor of the Chair of Pathological Anatomy, Belarusian State Medical University;
V.M. Tsarenkov – Doctor of Pharmaceutical Sciences, professor of the Chair of Pharmaceutical Technology, Belarusian State Medical University, https://orcid.org/0000-0001-8724-7273
E.N. Kalinichenko – Doctor of Chemical Sciences, Corresponding Member of the National Academy of Sciences, Deputy Director for Research and Innovation, Director of the Scientific and Production Center “ChemPharmSintez”, Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, https://orcid.org/0000-0002-9138-9271
T.S. Bozhok – Candidate of Chemical Sciences, senior researcher of the Scientific and Production Center “ChemPharmSintez”, Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus.
