DOI: https://doi.org/10.22263/2312-4156.2023.5.82
O.A. Sushinskaya
Quantitative determination of flavonoids amount in a liquid wormwood extract
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2023;22(5):82-89.
Abstract.
Objectives. To validate the methodology for quantitative determination of flavonoids amount in a liquid wormwood extract.
Material and methods. The object of the study was a 1:1 liquid extract of wormwood bitter herb obtained by the method of repercolation. Differential spectrophotometry was used to determine the amount of flavonoids in the liquid extract of wormwood. For the new object of research, the validation of the methodology for the quantitative determination of flavonoids amount was carried out according to the criteria of specificity, linearity, correctness, precision.
Results. Using spectrophotometry, the amount of flavonoids in the liquid extract of wormwood 1:1 was determined, which made up 1.54±0.04 mg/ml in terms of rutin. The norm of flavonoid amount in the liquid extract of wormwood 1:1 was proposed; it should be not less than 0.10%.
Conclusions. The technique is accurate and precise within the range of its application, the correlation coefficient and coefficients of variation for validation characteristics did not exceed the eligibility criteria, the specificity and linearity of the technique for a new extraction preparation from wormwood bitter herb was proved. The technique can be recommended for routine quality control of the obtained extract according to the indicator «Quantitative determination».
Keywords: liquid extract, wormwood, quantitative determination, flavonoids, spectrophotometry, validation.
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Information about authors:
O.A. Sushinskaya – Master of Pharmaceutical Sciences, postgraduate of the Chair of Pharmaceutical Technology, Belarusian State Medical University, https://orcid.org/0000-0002-6801-7285,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Olga A. Sushinskaya.
