DOI: https://doi.org/10.22263/2312-4156.2024.3.63
V.A. Malatok1, T.F. Chernykh2, S.E. Rzheussky1, A.A. Kuzmich1, D.S. Nebyshinets1
Determining the specific activity of medical hemostatic foam based on aluminum chloride
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Saint Petersburg State Chemical and Pharmaceutical University, Saint-Petersburg, Russian Federation
Vestnik VGMU. 2024;23(3):63-68.
Abstract.
In previous studies we have developed the medicine in the form of medical hemostatic foam based on aluminum chloride. The purpose of this work was to evaluate the specific activity of a medical hemostatic foam based on aluminum chloride by modelling parenchymal bleeding on the liver of rats with the withdrawal of animals on days 1, 3, 7, 14, 21, 30, which had been described in the Guidelines for conducting preclinical studies of medicines by Doctor of Medical Sciences, Professor A.N. Mironov. The time to achieve hemostasis and such postoperative complications as the presence of inflammation, signs of recurrent bleeding, adhesions and fibrin plaque were selected as evaluation criteria. The time of achieving hemostasis was determined as the time of removing the medicine from the wound surface. The recovery time of the animal’s activity after the performed operation was also assessed. It was determined that when using medical hemostatic foam based on aluminum chloride, hemostasis of parenchymal bleeding occurred on an average in 11.96± 6.56 s. The occurrence of fibrin plaque decreased by day 30, and its localization was limited to the area of the inflicted wound. It was established that the inflammatory process and the signs of recurrent bleeding were absent from the first day of observation. In 40 animals out of 72, an adhesive process developed, in 69 out of 72 rats, the activity was restored within 1 day.
Keywords: aluminum chloride, medical foam, parenchymal bleeding, hemostasis, hemostatic drugs.
Source of financing. Belarusian Republican Foundation for Basic Research. The research work was conducted within the frames of the BRFFR grant “Science-M” – 2022.
References
1. Obshcheros obshchestv org «Ros o-vo skoroi med pomoshchi». Clinical recommendations (protocol) on emergency medical care for abdominal, lower back trauma. Kazan, RF; 2014. 18 р. (In Russ.)
2. Bondarev GA, Lipatov VA, Severinov DA, Saakyan AR. Intraoperative tactics of local surgical hemostasis in trauma and planned operations on parenchymatous organs of the abdominal cavity. Vest Eksperim Klin Khirurgii. 2020;13(3):268-78. (In Russ.). doi: http://dx.doi.org/10.18499/2070-478X-2020-13-3-268-278
3. Aleksandrov VV, Maskin SS, Ermolaeva NK, Matyukhin VV. Conservative management of patients with closed trauma to parenchymatous organs of the abdominal cavity and retroperitoneum with retroperitoneal hemorrhage - indications, methodology and feasibility. Zhurn im NV Sklifosovskogo Neotlozh Med Pomoshch'. 2021;10(3):540-48. (In Russ.). doi: http://dx.doi.org/10.23934/2223-9022-2021-10-3-540-548
4. Kuchkarov MYu, Babadzhanov AKh, Abbullazhanov BR. Local hemostatics in parenchymatous organ surgery: biological and pharmacologic properties. Tibbiet Va Sport. 2023;(1):70-4. (In Russ.)
5. Bondarev GA, Lipatov VA, Lazarenko SV, Severinov DA, Saakyan AR. A survey of surgeons' opinions on the use of hemostatic application materials. Khirurgiya Zhurn im NI Pirogova. 2020;(8):61-8. (In Russ.). doi: http://dx.doi.org/10.17116/hirurgia202008161
6. Molotok VA, Rzheusskiy SE. Pharmaceutical development of medical hemostatic foam. Vestn Farmatsii. 2021;(4):22-5. (In Russ.)
7. Molotok VA, Rzheusskiy SE. Selection of auxiliary substances of medical styptic foam. Vestn Farmatsii. 2022;(3):56-64. (In Russ.)
8. Mironov AN, Bunyatyan ND, Vasilyev AN, Verstakova OL, Zhuravleva MV, Lepakhin VK, i dr, redkol. Guidelines for conducting preclinical studies of medicinal products. Ch 1. Moscow, RF: Grif i K; 2013. 944 р. (In Russ.)
9. Rebrova OYu. Statistical analysis of medical data. Application of STATISTICA application program package. Moscow, RF: Media Sfera; 2006. 312 р. (In Russ.)
10. Kirkwood BR, Sterne JAC. Essential medical statistics. Wiley-Blackwell; 2003. 2nd ed. 512 р.
11. Directive 2010/63/EU of the European Parliament and of the Council of the European Union of 22 September 2010 on the protection of animals used for scientific purposes [Internet]. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32010L0063. [Accessed 03th June 2024].
Submitted 19.03.2024
Accepted 14.06.2024
Information about authors:
V.A. Malatok – postgraduate of the Chair of Organization & Economy of Pharmacy, Vitebsk State Order of Peoples’ Friendship Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Veranika A. Malatok;
T.F. Chernykh – Doctor of Pharmaceutical Sciences, professor, head of the Chair of Microbiology, Saint Petersburg State Chemical and Pharmaceutical University,
S.E. Rzheussky – Candidate of Pharmaceutical Sciences, associate professor of the Chair of Organization & Economy of Pharmacy, Vitebsk State Order of Peoples’ Friendship Medical University;
A.A. Kuzmich – the fifth-year medical student, Vitebsk State Order of Peoples’ Friendship Medical University;
D.S. Nebyshinets – the fifth-year medical student, Vitebsk State Order of Peoples’ Friendship Medical University.
