DOI: https://doi.org/10.22263/2312-4156.2024.4.22
A.T. Shchastniy, A.S. Osochuk, S.S. Osochuk, A.F. Martsinkevich, N.N. Yarotskaya
The dynamics of changes in the spectrum of fatty acids of lipoprotein blood complexes and prostaglandins in patients who have taken tacrolimus in the late terms after kidney transplantation
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2024;23(4):22-30.
Abstract.
Objectives. To study the changes in the concentration of PGE2, PGE3 and the spectrum of fatty acids of native blood lipoprotein complexes in kidney allograft recipients in the long term after surgery.
Material and methods. The study group included 15 women (36-55 years old) and 15 men (36-60 years old) in 1, 3 and 5 years after kidney transplantation. The control group consisted of 15 healthy women and men of the same age. Native blood lipoprotein complexes were isolated by means of preparative ultracentrifugation. The amount of prostaglandin E2(PGE2) and E3(PGE3) was determined by enzyme immunoassay. The spectrum of fatty acids was determined using gas chromatography.
Results. The amount of PGE2 and the PGE2/PGE3 ratio at all postoperative periods were higher than in the group of healthy people. An assessment of the fatty acid spectrum of lipoprotein complexes showed that the ratio of saturated to polyunsaturated fatty acids (SFA/PUFA) was reduced at all times after kidney transplantation.
In the fatty acid spectrum of VLDL, an increase in the content of C18:2n6 and C20:4n6 was determined during all periods of the study, and the content of C20:3n3 increased in 3 years after surgery; a decrease in C18:3n6 in 3 years after surgery in men compared to controls and women after kidney transplantation and a decrease in C22:6n3 levels in 3 and 5 years after surgery. In the fatty acid spectrum of LDL and HDL, a decrease in C18:3n6, C18:3n3 and C20:3n6 and an increase in C18:2n6c were determined at all times after surgery.
Conclusions. The fact of an increase in the production of pro-inflammatory PGE2 and the PGE2/PGE3 ratio may be the evidence of the presence of an inflammatory process. The detected decrease in the SFA/PUFA ratio can increase the fluidity of lipoprotein blood complexes, change the conformation of their apoproteins and modify their functional activity. There is a deficiency and imbalance of essential PUFAs in the composition of all lipoprotein blood complexes, which may cause a change in the structure of transport of essential PUFAs with a weakening of their inclusion in the HDL phospholipids and an increase in the inclusion of VLDL in the triglycerides.
Keywords: kidney transplantation, lipoprotein complexes, fatty acids, tacrolimus, prostaglandins.
Information about the source of support in the form of grants, equipment, medicinal agents. The research was carried out within the frames of the State Research Program (GPNI) “Translational medicine”, subprogram 4.2 “Fundamental aspects of medical science”, task 3.37 “To study the condition of lipid transport and immune systems of patients with kidney transplantation and to substantiate the approaches to their correction”, State Registration No. 20220305 dated 16.03.2022. The authors didn’t get any financial support on the part of the manufacturing companies of medicinal agents.
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Submitted 20.06.2024
Accepted 28.08.2024
Information about authors:
A.T. Shchastniy – Doctor of Medical Sciences, professor, head of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0003-2796-4240
A.S. Osochuk – lecturer of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-5942-3601,
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Alexander S. Osochuk;
S.S. Osochuk – Doctor of Medical Sciences, professor, head of the research laboratory, Vitebsk State Order of Peoples’ Friendship Medical University, ORCID: https://orcid.org/0000-0003-2074-3832
A.F. Martsinkevich – Candidate of Biological Sciences, associate professor of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0003-3655-4489
N.N. Yarotskaya – Candidate of Biological Sciences, associate professor of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https:// 0000-0002-2493-7653.
