DOI: https://doi.org/10.22263/2312-4156.2024.4.79
V.M. Semenov1, T.I. Dmitrachenko1, S.K. Yahorau1, A.N. Kizimenka1, I.A. Lyatos1, S.K. Ziankova1, K.M. Kubrakou1, E.A. Matusevich2, A.M. Kashtanov2
TTV DNA level as a marker of cellular immune intensity response in patients with generalized bacterial infection
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Vitebsk Regional Clinical Hospital, Vitebsk, Republic of Belarus
Vestnik VGMU. 2024;23(4):79-86.
Abstract.
TTV DNA is detected in the vast majority of healthy individuals as well as in patients with infectious pathology, regardless of its severity, and makes up 85.4% in patients with severe bacterial infection.
Objectives. To evaluate the intensity of the cellular immune response in patients with bacteremia at different levels of TTV DNA in the blood plasma. The average level of viral load (M) in patients with bacteremia was 28,748 copies/ml (CI 95%; 3620; 53876 copies/ml), which significantly exceeds similar values in healthy individuals. At the same time, in healthy individuals the level of viral load does not exceed 18,475 copies/ml. In patients with severe bacterial infection, the functional activity of T- and B-cell immunity in response to activation by mitogens is significantly lower compared to healthy individuals (p<0.001). The level of TTV DNA in the blood plasma of patients with generalized bacterial infection reflects the intensity of the immune response to mitogens in the RBTL and can be used as an additional marker for assessing the immune status. A TTV DNA level exceeding 2300 copies/ml can serve as a fairly specific additional criterion for reducing the intensity of the immune response and the risk of a more severe course of the disease. The determination of the TTV DNA level can be used as a marker for assessing the immune status and the presence of immunosuppression in patients with bacteremia.
Keywords: cellular immune response, pathogens of bacterial infections (K. pneumoniae, P. aeruginosa, A. baumannii), TTV DNA.
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Submitted 04.03.2024
Accepted 28.08.2024
Information about authors:
V.M. Semenov – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-7029-9226
T.I. Dmitrachenko – Doctor of Medical Sciences, professor of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University; https://orcid.org/0000-0002-5537-3459
S.K. Yahorau – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0001-7352-9368
A.N. Kizimenka – Candidate of Medical Sciences, associate professor of the Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
I.A. Lyatos – Candidate of Medical Sciences, associate professor, head of the Military Chair, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0009-0008-9011-2028
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Igor A. Lyatos;
S.K. Ziankova – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-7446-2684
K.M. Kubrakou – Doctor of Medical Sciences, associate professor, professor of the Chair of Neurology & Neurosurgery, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0001-6723-0589
E.A. Matusevich – Candidate of Medical Sciences, chief physician, Vitebsk Regional Clinical Hospital;
A.M. Kashtanov – postgraduate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University, deputy chief physician for medical affairs, Vitebsk Regional Clinical Hospital.
