DOI: https://doi.org/10.22263/2312-4156.2024.5.102
S.S. Malchenkova, N.S. Golyak, Z.F. Tsyrkunova, N.N. Berdnik
Antibacterial properties of sodium thiosulfate aqueous solutions
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2024;23(5):102-111.
Abstract.
Objectives. To identify the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in vitro of the aqueous solution of sodium thiosulfate against standard test cultures of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans.
Material and methods. The tests were carried out using the method of serial microdilutions with further inoculation on the solid culture media. The presence or absence of bacterial growth was assessed by measuring the optical density of the suspension in the wells of the microplate, as well as by changing the medium color after adding the indicator triphenyltetrazolium chloride.
Results. MIC of sodium thiosulfate aqueous solution against Pseudomonas aeruginosa was 200 mg/ml; Staphylococcus aureus – 100 mg/ml; Escherichia coli – 200 mg/ml. MBC of sodium thiosulfate aqueous solution against Escherichia coli was 250 mg/ml; Candida albicans – 37,50 mg/ml.
Conclusions. Sodium thiosulfate aqueous solutions can not only inhibit the growth of microorganisms, but also have a bactericidal effect on them. This property of sodium thiosulfate affects the microbiological purity of extemporaneous solutions, and therefore their shelf life.
Keywords: sodium thiosulfate, minimum inhibitory concentration, minimum bactericidal concentration, compounding, extemporaneous formulation.
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Submitted 22.05.2024
Accepted 18.10.2024
Information about authors:
S.S. Malchenkova – postgraduate, senior lecturer of the Chair of Pharmaceutical Technology, Belarusian State Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Svetlana S. Malchenkova;
N.S. Golyak – Candidate of Pharmaceutical Sciences, associate professor, head of the Chair of Pharmaceutical Technology, Belarusian State Medical University, https://orcid.org/0000-0002-4904-6523
Z.F. Tsyrkunova – Candidate of Biological Sciences, head of the Laboratory of nosocomial infections of the Research Department of the Research Institute of Experimental and Clinical Medicine, Belarusian State Medical University;
N.N. Berdnik – researcher of the Laboratory of nosocomial infections of the Research Department of the Research Institute of Experimental and Clinical Medicine, Belarusian State Medical University;
