DOI: https://doi.org/10.22263/2312-4156.2024.5.31
T.N. Lepteeva, S.A. Senkovich, M.V. Masko, J.J. Bulinska, A.N. Ugalev
The ability of blood serum to destroy the biofilm matrix and suppress bacteria metabolic activity in the native biofilm in patients with rheumatoid arthritis and purulent inflammatory diseases
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2024;23(5):31-41.
Abstract.
The risk of developing infectious processes in rheumatoid arthritis is significantly increased compared to healthy people. Bacteria that exist in biofilms play a crucial role in the development of infections. The study of the macroorganism ability to influence the bacterial biofilm may contribute to the search for new prognostic biomarkers of the infectious process in rheumatoid arthritis.
Objectives. To perform the comparative assessment of the blood sera ability to suppress the metabolic activity of native biofilm bacteria and destroy the biofilm exopolymer matrix in patients with rheumatoid arthritis, purulent inflammatory diseases and in healthy individuals.
Material and methods. The blood sera of 28 patients with rheumatoid arthritis, 20 patients with purulent inflammatory diseases and 48 healthy individuals were examined. Previously developed methods were used to evaluate the ability of blood serum to suppress the metabolism of the native biofilm bacteria and destroy the exopolymer biofilm matrix.
Results. In patients with rheumatoid arthritis and purulent inflammatory diseases, the ability of serum to suppress the metabolic activity of biofilms and destroy the biofilm matrix was significantly lower than in healthy individuals (p<0.05). Tests determining the ability of serum to destroy the biofilm matrix make it possible to differentiate between patients with rheumatoid arthritis and healthy individuals (sensitivity – 92.86%, specificity – 82.14%) as well as between patients with purulent inflammatory diseases and healthy individuals (sensitivity – 80.00%, specificity – 80.00%).
Conclusions. A reduced ability of the blood serum to suppress the metabolic activity of the native biofilm bacteria and destroy the biofilm exopolymer matrix in patients with rheumatoid arthritis and purulent inflammatory diseases has been established. Further research in this area is required to determine the pathogenetic mechanisms and the diagnostic application of the obtained data.
Keywords: rheumatoid arthritis, purulent inflammatory diseases, biofilm, metabolic activity of bacteria, exopolymer matrix of biofilm.
Acknowledgments. The authors express their sincere gratitude to the reviewers of the article and the editorial board.
The sources of funding. The study was carried out as a part of the research “The influence of the immunity humoral factors on the bacterial biofilm” with financial assistance of BRFFR grant under the Treaty, dated May 2, 2023 М23М-108.
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Submitted 02.08.2024
Accepted 18.10.2024
Information about authors:
T.N. Lepteeva – lecture of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University, https://orcid.org/0000-0002-5364-9909,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Taisiya N. Lepteeva;
S. A. Senkovich – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University;
M.V. Masko – Doctor of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University;
J.J. Bulinska – teacher-trainee of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University;
A.N. Ugalev – senior lecturer of the Chair of General and Orthopedic Dentistry with the course of the Faculty for Advanced Training and Retraining,Vitebsk State Order of Peoples’ Friendship Medical University.
