DOI: https://doi.org/10.22263/2312-4156.2024.6.133
R.I. Lukashou, N.S. Gurina
Effect of the purple coneflower herb heat pre-treatment on the yield of hydroxycinnamic acids during extraction
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2024;23(6):133-140.
Abstract.
Objectives. To investigate the effect of heat pre-treatment of purple coneflower herb on the yield of hydroxycinnamic acids (HCAs) during extraction.
Material and methods. The object of the study was purple coneflower herb. The total HCAs content was determined quantitatively by spectrophotometry. The qualitative composition of HCAs was determined by high-performance liquid chromatography by comparing with standards. Heat pre-treatment of the herb was carried out in the air sterilizer at different temperatures. The HCAs content was compared with the activity of polyphenol oxidase.
Results. The temperature, duration of pre-treatment, presence of packaging, thickness of the processed medicinal plant raw material layer and degree of medicinal plant raw material grinding statistically significantly affect the HCAs yield during extraction. With 60ºС pre-treatment temperature in the package, 0.5-2 h duration, 2-4 cm thickness of the layer of processed medicinal plant raw material powder, the HCAs yield during extraction increases by 2.8 times, the qualitative composition and ratio of the main HCAs do not change. Polyphenol oxidase activity significantly influences the yield of HCAs during extraction from medicinal plant raw materials subjected to heat treatment in the temperature range from 40 to 100ºС. A different nature of the temperature and time dependence of the of HCAs yield during extraction for thermally treated and native medicinal plant raw materials has been revealed; from heat pre-treated medicinal plant raw materials, the yield during extraction in a boiling water bath for 1.5-2 hours is almost three times greater than the HCAs.
Conclusions. The criteria for heat pre-treatment of purple coneflower herb have been experimentally substantiated and selected, which increase the yield of HCAs during extraction without changing their qualitative composition and ratio. These parameters allow obtaining HCAs -enriched extracts from purple coneflower herb.
Keywords: purple coneflower, herb, hydroxycinnamic acids, heat pre-treatment, increase of extraction, polyphenol oxidase.
Sources of financing: The work was carried out as part of task 2.2.3 Production and Standardisation Extracts with an Increased Content of Biologically Active Substances within the framework of state research programme 2 Chemical Processes, Reagents and Technologies, Bioregulators and Bioorganic Chemistry and sub-programme 2.2 Synthesis and Targeted Modification of Bioprocess Regulators (Bioregulators).
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Submitted 19.08.2024
Accepted 04.12.2024
Information about authors:
R.I. Lukashou – Candidate of Pharmaceutical Sciences, associate professor, head of the Chair of Pharmaceutical Chemistry, Belarusian State Medical University, https://orcid.org/0000-0001-5234-6319,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Lukashou Raman Iharavich;
N.S. Gurina – Doctor of Biological Sciences, professor, dean of the pharmaceutical faculty, Belarusian State Medical University, https://orcid.org/0009-0009-9150-5728
