DOI: https://doi.org/10.22263/2312-4156.2025.2.114
L.G. Lappo, V.A. Hrynchak, S.I. Sychik
In vitro hemocompatibility of medical devices with different duration and type of contact with a patient
Republican Center for Hygiene, Epidemiology and Public Health, Minsk, Republic of Belarus
Vestnik VGMU. 2025;24(2):114-123.
Abstract.
The aim of the work was to study the in vitro hemocompatibility of medical devices with different duration and type of contact with a patient using a test model of the artificial blood flow. An in vitro test model of the artificial blood flow with the whole heparinized human blood was used. After incubation in the test model for 20, 60 and 120 minutes, the following blood parameters were determined: the number of platelets and leukocytes, the content of the prothrombin fragment F1+2, thrombin-antithrombin complex III, beta-thromboglobulin, thromboxane B2 and protein C3a of the complement system.
All the studied medical devices after incubation for 20 minutes were found to have high hemocompatibility. With an increase in the incubation time to 120 minutes, the compatibility of medical device samples with blood decreased. After 60 minutes of incubation in a test model of the artificial blood flow of the medical devices including intrauterine device (polyethylene), surgical mesh (polypropylene) and needle (stainless steel) statistically significant changes in the morphofunctional parameters of blood were observed, which underwent even more pronounced negative changes after increasing the incubation time to 120 minutes. For devices having constant contact with the internal body environment, high compatibility with blood was established, which did not depend on the incubation time in the in vitro test model of the artificial blood flow.
Keywords: hemocompatibility, medical devices, coagulation markers, platelet activation, in vitro test model, whole human blood.
The sources of funding. The study was carried out within the framework of the task 04.08. “Develop a method for assessing the in vitro hemocompatibility of medical devices based on a test model of artificial blood flow” of the State Scientific and Technical Program “Scientific and Technical Support for the Quality and Availability of Medical Services” for 2021-2025, subprogram “Safety of the Human Environment” (state registration number 20220371 dated March 28, 2022).
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Submitted 06.02.2025
Accepted 14.04.2025
Information about authors:
Lidziya G. Lappo ‒ rresearcher, laboratory of preventive and environmental toxicology Research Institute of Hygiene, Toxicology, Epidemiology, Virology and Microbiology, Republican Center for Hygiene, Epidemiology and Public Health, https://orcid.org/0009-0009-0312-3982, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
V.A. Hrynchak ‒ Candidate of Medical Sciences, head of the laboratory of applied toxicology and safety of medical products, Research Institute of Hygiene, Toxicology, Epidemiology, Virology and Microbiology, Republican Center for Hygiene, Epidemiology and Public Health, https://orcid.org/0000-0002-4119-1793
S.I. Sychik ‒ Candidate of Medical Sciences, Associate Professor, Director Research Institute of Hygiene, Toxicology, Epidemiology, Virology and Microbiology, Republican Center for Hygiene, Epidemiology and Public Health, https://orcid.org/0000-0002-5493-9799
