DOI: https://doi.org/10.22263/2312-4156.2025.2.29
A.F. Raduta1, A.B. Astrowskaja2, T.A. Baradzina1, A.G. Shlyahtun1, A.A. Astrowski1
The development of dermal-cartilaginous autografts on the surface of a full-thickness skin defect in rats
1Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus
2Grodno State Medical University”, Grodno, Republic of Belarus
Vestnik VGMU. 2025;24(2):29-38.
Abstract.
Objectives. To investigate the processes occurring on the receptive field during 600 days with dermal-cartilaginous allografts made from the auricles of laboratory rats.
Material and methods. The study was performed on male Wistar rats in which a full-thickness skin defect was created on the skin in the interscapular region inside a plastic security fixation. One auricle was taken from each animal, from the skin of the inner surface of which (containing cartilaginous tissue) several pieces were cut. The latter were distributed on the surface of the full-thickness skin defect of other rats. After 5-600 days, the animals were killed. Tissues along with allografts were excised from the receptive field. Histological and electron microscopic techniques were used to analyze and describe the structures present on the receptive field.
Results and conclusions. After allotransplantation of skin-cartilage grafts, primary engraftment of auricular grafts on the receptive field was observed, followed by rejection of all other graft tissues except cartilage. The cartilage tissue, which was not damaged during graft fabrication, remained viable in a virtually intact state under the skin and scar tissue of the recipient for more than 600 days. In the areas with damaged lower row of differentiated cells of the chondrocytic layer, activation of intercellular matrix synthesis and proliferation of cambial chondrocytes were occasionally observed between 5-10 days after allotransplantation. At the edges of allotransplanted chondrocyte layers, as well as on those parts of the latter, which were crumpled or layered on each other due to contraction, 200 and more days after allotransplantation atrophy manifestations were revealed.
Keywords: rats, allotransplantation, skin-cartilage grafts, full-thickness skin defect, auricle, cartilage tissue.
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Submitted 17.09.2024
Accepted 14.04.2025
Information about authors:
Alena F. Raduta – scientific secretary, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0001-8020-1838, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
A.B. Astrowskaja – Candidate of Medical Sciences, associate professor of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, Grodno State Medical University, https://orcid.org/0000-0003-3513-2014;
T.A. Baradzina – Researcher, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0009-0004-9607-7471;
A.G. Shlyahtun – Head of the Sectoral Laboratory, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0002-7618-9589;
A.A. Astrowski – Doctor of Medical Sciences, professor, Leading Researcher, Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0001-8647-3922.
