DOI: https://doi.org/10.22263/2312-4156.2025.2.9
V.L. Moroz, E.B. Belonovskaya, A.V. Romanchuk, I.A. Kuzmitskaya, V.Ch. Polubok, T.A. Borodina, A.A. Astrowski, A.H. Shlyahtun, L.I. Nadolnik
Immunomodulatory and anti-inflammatory properties of plant polyphenols (chlorogenic acid, naringin) in chronic alcohol intoxication
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, Grodno, Republic of Belarus
Vestnik VGMU. 2025;24(2):9-19.
Abstract.
Chronic ethanol consumption is the cause of developing socially significant human diseases. The development of drugs and biologically active supplements is aimed at reducing alcohol dependence, as well as treating diseases induced by its excessive consumption.
Objectives. To investigate immunomodulatory and anti-inflammatory properties of plant polyphenols (chlorogenic acid (CGA), naringin (NG)) and their compositions in rats subjected to chronic alcohol intoxication as well as in vitro studies.
Material and methods. CGA (40 mg/kg) and NG (40 mg/kg) as well as the composition of CGA and NG (40 mg/kg+20 mg/kg) were tested on the model of chronic alcohol intoxication (CAI, consumption of 10% ethanol solution as the only source of liquid): against the background of ethanol consumption (28 weeks) and after its withdrawal. Inflammatory markers, phagocytic (PI, %) and metabolic activity of neutrophils were determined in the blood serum; the degree of leukocyte infiltration was assessed in the liver and the heart.
Results. The introduction of CGA and NG during 4 weeks increased PI to maximum values and decreased the metabolic activity of neutrophils. The best immunomodulatory properties are shown for the composition of CGA + NG. The anti-inflammatory activity of polyphenols was manifested by the decrease in the number of foci of leukocyte infiltration, their area in the liver and the heart, myeloperoxidase activity, the level of TNF-1α in the blood, and normalization of the blood count. In vitro studies have shown that the effects of the composition of CGC+NG (2:1) most effectively restored reduced FI and optimized the increased metabolic activity of neutrophils.
Conclusions. CGA and NG, their composition is fascinating for the correction of secondary immunodeficiencies in chronic alcohol intoxication, as well as reducing the degree of alcoholic damage to the heart and the liver.
Keywords: chronic alcohol intoxication, immunomodulatory properties, anti-inflammatory properties, TNF-1α, chlorogenic acid, naringin.
The sources of funding. The study was performed as a part of the program 4.1.5 State Scientific Research Programs “Translational Medicine”, subprogram “Experimental Medicine”, 2021-2025.
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Submitted 30.12.2024
Accepted 14.04.2025
Information about authors:
V.L. Moroz – Senior Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0009-0001-3914-6985
E.B. Belonovskaya – Senior Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0000-0002-8354-4606
A.V. Romanchuk – Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0000-0003-0318-352X
I.A. Kuzmitskaya – Senior Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0009-0001-6362-5679
V.Ch. Polubok – Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0000-0002-9418-7069
T.A. Borodina – Researcher, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0009-0004-9607-7471
A.A. Astrowski – Doctor of Medical Sciences, professor, https://orcid.org/0000-0001-8647-3922
A.H. Shlyahtun, – Head of the Industry Laboratory, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus”, https://orcid.org/0000-0002-7618-9589
Liliya I. Nadolnik – Doctor of Biological Sciences, associate professor, head of the Department of Preclinical and Experimental Research, Republican Research Unitary Enterprise “Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, https://orcid.org/0000-0002-0363-7969, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра..
