DOI: https://doi.org/10.22263/2312-4156.2025.3.115
A.S. Bakun
The development of a technique for quantitative determination of phenolic compounds in Galega officinalis herb, identification of its pharmacological activity
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2025;24(3):115-122.
Abstract.
Objectives. To establish optimal conditions for extracting phenolic compounds from Galega officinalis herb and to study its pharmacological activity.
Material and methods. The object of the study was Galega officinalis herb, harvested in Vitebsk region during the mass flowering phase.
Quantitative determination of phenolic compounds in the herb of Galega officinalis was carried out by a spectrophotometric method using a complexation reaction with the Folin-Ciocalteu reagent. Antioxidant activity (in vitro) was studied by a colorimetric method based on the reaction with 1,1-diphenyl-2-picrylhydrazyl. Hypoglycemic effect was studied on the model of oral sugar load.
Results. As a result of the research, optimal conditions for the extraction of phenolic compounds were determined and a method for their quantitative determination in the herb of Galega officinalis was developed.
All extracts from the herb Galega officinalis have antioxidant activity: the percentage of inhibition of DPPH free radicals varied within the range of 38.3% – 77.7%. It has been proven that the aqueous extract from the herb Galega officinalis at a dose of 500 mg/kg has a hypoglycemic effect (no statistically significant differences with the effect of metformin were found).
Keywords: Galega officinalis, optimal conditions, phenolic compounds, antioxidant activity, hypoglycemic effect.
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Submitted 28.03.2025
Accepted 13.06.2025
Information about authors:
Anastasiya S. Bakun – postgraduate of the Chair of Organization of Pharmacy with the Course of the Faculty for advanced training and retraining Belarusian State Medical University, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра..
