Menu

A+ A A-

Download article

DOI: https://doi.org/10.22263/2312-4156.2025.4.42

L.M. Miadzvedzeva, N.K. Karalkova, N.N. Yarotskaya
Evaluation of some structural and functional changes in the erythrocyte membrane in patients with age-related macular degeneration. A clinician’s view
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2025;24(4):42-48.

Abstract.
Objectives. To determine the content of total protein and lipids in erythrocyte membranes in patients with age-related macular degeneration (AMD).
Material and methods. The study group consisted of patients with the “wet” form of AMD, and the comparison group without AMD. The first group included 76 patients (mean age was 73 years), 72% women and 28% men, and the second group included 50 people (mean age was 70 years), 60% women and 40% men. The ophthalmological examination was performed, venous blood was taken and erythrocyte membranes were isolated using the Dodge method. Total cholesterol was determined using the Zlatkis-Zak method. The content of total phospholipids was measured by the optical density of the standard. Protein concentration was determined using the Lowry method.
Results. It was revealed that the groups differed significantly in the content of total protein (TP) in erythrocyte membranes (p=0.000005). The ratio of cholesterol to total protein (Chol/TP) in the study group was significantly lower, p=0.00039, phospholipids to total protein (PL/TP), p=0.021, was also lower, and cholesterol to phospholipids (Chol/PL) was greater than 0.9 in both groups (p=0.78), which corresponds to the age-related changes. 
Discussions. Having studied the protein-lipid profile of the erythrocyte membrane of patients with AMD, it was found that the content of total protein in the membrane was 1.5 times higher, the ratio of Chol/TP and PL/TP was significantly lower. The expected difference in the content of Chol, PL and the PL/Chol index was not revealed. Changes in the membrane structure naturally lead to functional disorders in the erythrocyte itself, which entails a number of possible systemic changes. AMD should not be considered as a local process associated with changes only in the retinal pigment epithelium, Bruch’s membrane and choriocapillaris. The onset of the disease, its course, can serve as a risk factor for systemic disorders and requires further study.
Keywords: age-related macular degeneration, erythrocyte membrane, pathogenesis, oxidative stress.

References

1. Plyaskina US, Fomina AV. Epidemiological aspects of age-related macular degeneration and renal cataracts in Russia and the world. Sovremennye Problemy Zdravoohranenija Medicinskoj Statistiki. 2024;(3):713-730. (In Russ.). doi: http://dx.doi.org/10.24412/2312-2935-2024-3-713-730
2. Mendanha SA, Anjos JLV, Silva AHM, Alonso A. Electron paramagnetic resonance study of lipid and protein membrane components of erythrocytes oxidized with hydrogen peroxide. Brazilian Journal of Medical and Biological Research. 2012 Jun;45(6):473-481. doi: http://dx.doi.org/10.1590/s0100-879x2012007500050 
3. Lemarechal H, Allanore Y, Chenevier-Gobeaux C, Kahan A, Ekindjian OG, Borderie D. Serum protein oxidation in patients with rheumatoid arthritis and effects of infliximab therapy. Clinica Chimica Acta. 2006 Oct;372(1-2):147-153. doi: http://dx.doi.org/10.1016/j.cca.2006.04.002 
4. Ermakova NA, Rabdanova OTs. Main etiological factors and pathogenetic mechanisms of age-related macular degeneration. Klinicheskaja Oftal'mologija. 2007;8(3):125-128. (In Russ.).
5. Dodge J, Mitchell C, Hanahan D. The preparation and chemical characteristics of hemoglobin free ghosts of erythrocytes. Archives of Biochemistry and Biophysics. 1963 Jan:100:119-130. doi: http://dx.doi.org/10.1016/0003-9861(63)90042-0 
6. Troshkina NA, Tsirkin VI, Dvoryanskiy SA. Erythrocyte: structure and functions of its membranes. Vyatskii Meditsinskii Vestnik. 2007;(2-3):32-40. (In Russ.).
7. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Acta. 2003 Mar;329(1-2):23-38. doi: http://dx.doi.org/10.1016/s0009-8981(03)00003-2 
8. Li J, Wang D, Pottenburgh J, Bower AJ, Asanad S, Lai EW, et al. Visualization of erythrocyte stasis in the living human eye in health and disease. iScience. 2022 Dec 7;26(1):105755. doi: http://dx.doi.org/10.1016/j.isci.2022.105755 
9. Zanón-Moreno V, Domingo Pedrol JC, Sanz-González SM, Raga-Cervera J, Salazar-Corral J, Pinazo-Durán MD. Feasibility Study of a Docosahexaenoic Acid-Optimized Nutraceutical Formulation on the Macular Levels of Lutein in a Healthy Mediterranean Population. Ophthalmic Research. 2021;64(6):1068-1076. doi: http://dx.doi.org/10.1159/000509439 
10. Aggarwal S, Moir J, Hyman Metformin MJ, Kaufmann GT, Flores A, Hariprasad SM, et al. Use and Age-Related Macular Degeneration in Patients Without Diabetes. JAMA Ophthalmology. 2024 Jan;142(1):53-57. doi: http://dx.doi.org/10.1001/jamaophthalmol.2023.5478 
11. Toppila V, Ranta-Aho S, Kaarniranta K, Hytti M, Kauppinen A. Metformin Alleviates Inflammation and Induces Mitophagy in Human Retinal Pigment Epithelium Cells Suffering from Mitochondrial Damage. Cells. 2024 Aug;13(17):1433. doi: http://dx.doi.org/10.3390/cells13171433 
12. Mkrtumyan AM, Markova TN, Ovchinnikova MA, Ivanova IA, Kuzmenko KV. AMF-dependent protein kinase activator. Known and new mechanisms of action. Sakharnyi Diabet. 2023;26(6):585-595. (In Russ.). doi: http://dx.doi.org/10.14341/DM13044
13. Moir J, Hyman MJ, Gonnah R, Flores A, Hariprasad SM, Skondra D. The Association Between Metformin Use and New-Onset ICD Coding of Geographic Atrophy. Investigative Ophthalmology and Visual Science. 2024 Mar;65(3):23. doi: http://dx.doi.org/10.1167/iovs.65.3.23 
14. Han J, Li Y, Liu X, Zhou T, Sun H, Edwards P, et al. Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One. 2018 Mar;13(3):e0193031. doi: http://dx.doi.org/10.1371/journal.pone.0193031
15. Lee J, Suh HS, Hwang IC. The Relationship between Age-Related Macular Degeneration and Cardiovascular Disease: A Meta-Analysis. Iranian Journal of Public Health. 2021 Feb;50(2):219-231. doi: http://dx.doi.org/10.18502/ijph.v50i2.5334 

Submitted 18.07.2025
Accepted 22.08.2025

Information about authors:
Liudmila M. Miadzvedzeva – senior lecturer of the Chair of Ophthalmology & Otorhinolaryngology, Vitebsk State Order of Peoples’ Friendship Medical University, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
N.K. Karalkova – Candidate of Medical Sciences, associate professor, head of the Chair of Ophthalmology & Otorhinolaryngology, Vitebsk State Order of Peoples’ Friendship Medical University;
Natalia N. Yarotskaya – Candidate of Medical Sciences, associate professor of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.

Поиск по сайту