DOI: https://doi.org/10.22263/2312-4156.2025.6.26
А.S. Doronkina, А.R. Gavrilchik, A.A. Rudak
Antinociceptive effects of palmitoylethanolamide and stearoylethanolamide upon PPARγ receptor blockade in rats with peripheral neuropathy
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk, Republic of Belarus
Vestnik VGMU. 2025;24(6):26-36.
Abstract.
Objectives. To investigate the role of PPARγ receptors in realization of the antinociceptive effects of PEA and SEA in rats with peripheral neuropathy.
Material and methods. Interaction of palmitoylethanolamide and stearoylethanolamide with the PPARγ receptor was studied using the molecular computer modeling to evaluate the binding affinity and stability of these complexes. The antinociceptive effects of acylethanolamides on PPARγ receptor blockade in rats with peripheral mononeuropathy were studied.
Results. Computer molecular modeling between acylethanolamides and the PPARγ receptor has shown the formation of stable complexes characterized by the formation of intermolecular hydrogen bonds and a network of hydrophobic interactions. The obtained results substantiate the feasibility of an experimental assessment of the involvement of PPARγ receptors in the antinociceptive effects of acylethanolamides. Administration of palmitoylethanolamide and stearoylethanolamide to rats with peripheral mononeuropathy with PPARγ receptor blockade reduced the threshold of nociceptive reactions and the latent period of nociceptive reactions compared with the values of the group administered with acylethanolamides without blockade. Single administration of palmitoylethanolamide with PPARγ receptor blockade reduced the area and intensity of the ipsilateral limb imprint in animals with neuropathy compared with administration of the test substance without PPARγ receptor blockade.
Conclusions. The realization of the antinociceptive effects of palmitoylethanolamide and stearoylethanamide in response to thermal and mechanical stimuli is mediated by PPARγ receptors.
Keywords: palmitoylethanolamide, stearoylethanolamide, gait patterns, neuropathy, acylethanolamides, nuclear PPARγ receptors.
The sources of funding. The research work was carried out with the financial support of the Belarusian Republican Foundation for Fundamental Research (BRFFR. № М24М-066).
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Submitted 06.10.2025
Accepted 10.12.2025
Information about authors:
Anastasya S. Doronkina – Candidate of Biological Sciences, senior researcher at the Center of pain research, Institute of Physiology of the National Academy of Sciences of Belarus, https://orcid.org/0000-0001-8914-5166, е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
A.R. Gavrilchik ‒ junior researcher at the Center of pain research, Institute of Physiology of the National Academy of Sciences of Belarus;
A.A. Rudak ‒ postgraduate student, junior researcher at the Center of pain research, Institute of Physiology of the National Academy of Sciences of Belarus, https://orcid.org/0009-0001-3698-5935
