DOI: https://doi.org/10.22263/2312-4156.2026.2.104
V.A. Tsiarletskaya, R.I. Lukashou
Evaluation of acute toxicity of hydroalcoholic extracts of Lamium album, Lamium purpureum, Lamium maculatum and Lamium galeobdolon herb
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2026;25(2):104-112.
Abstract.
Objectives. To conduct a preclinical evaluation of the acute toxicity of dry residues obtained from aqueous–ethanolic extracts of Lamium album, Lamium purpureum, Lamium maculatum, and Lamium galeobdolon following a single oral administration.
Material and methods. The study was performed on 50 Wistar rats of both sexes in accordance with OECD (Organisation for Economic Co-operation and Development) Guideline No. 423, Acute Toxicity – Acute Toxic Class Method, using a limit dose of 5000 mg/kg body weight. Over a 14-day observation period, clinical signs of toxicity, mortality, and body weight changes were recorded. In addition, a macroscopic examination of internal organs was conducted, and their absolute and relative weights were determined.
Results. No mortality was observed in either the control or treatment groups. There were no signs of systemic toxicity or abnormalities in behavior, respiration, autonomic functions, or motor coordination. Body weight gain in treated animals followed normal physiological growth patterns and did not differ from that of the control group. No macroscopic pathological changes in organs were detected. Furthermore, no statistically significant differences in absolute or relative organ weights were found between treated and control groups in either males or females (p>0.05).
Conclusions. According to the criteria of the Globally Harmonized System of Classification and Labelling of Chemicals (GHS), the dry residues of aqueous–ethanolic extracts of L. album, L. purpureum, L. maculatum, and L. galeobdolon can be classified as “not classified” (LD₅₀>5000 mg/kg). The results indicate a low level of acute toxicity of extracts from plants of the genus Lamium following single oral administration.
Keywords: acute toxicity; Lamium album; Lamium purpureum; Lamium maculatum; Lamium galeobdolon; preclinical studies; laboratory animals.
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Submitted 22.01.2026
Accepted 14.04.2026
Information about authors:
Victoryia A. Tsiarletskaya – postgraduate student, assistant of the Chair of Pharmaceutical Chemistry with a course for Advanced Training and Retraining, Belarusian State Medical University, https://orcid.org/0009-0000-8848-4617, email: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
R.I. Lukashou – Candidate of Pharmaceutical Sciences, associate professor, head of the Chair of Pharmaceutical Chemistry with a course for Advanced Training and Retraining, Belarusian State Medical University, https://orcid.org/0000-0001-5234-6319
