DOI: https://doi.org/10.22263/2312-4156.2026.3.115
V.A. Tsiarletskaya1, R.I. Lukashou1, A.V. Butenka2, Z.B. Kvachova2, H.G. Paleshka2
Morphofunctional and immunophenotypic characteristics of human dermal fibroblasts under the influence of phytoextracts of plants of the genus Lamium
1Belarusian State Medical University, Minsk, Republic of Belarus
2Institute of Biophysics and Cell Engineering NAS of Belarus, Minsk, Republic of Belarus
Vestnik VGMU. 2026;25(3):115-127.
Abstract.
Objectives. To determine the maximally tolerated concentrations of Lamium album, Lamium purpureum and Lamium maculatum phytoextracts for the human dermal fibroblasts, as well as their effects on the morphofunctional and immunophenotypic characteristics of the cells.
Material and methods. The study was performed on the human dermal fibroblast cultures at passages 3–5. Hydroalcoholic extracts of Lamium plants were added to the cultures at concentrations of 0.25, 0.5, and 1.5 μM, calculated as chlorogenic acid. Cell viability, monolayer formation and morphology, proliferation index, and the expression of surface and intracellular markers were assessed using flow cytofluorimetry. Stress-induced cellular senescence was modeled using H2O2; acid β-galactosidase activity was determined as a senescence marker. Statistical data processing was performed using nonparametric tests.
Results. Extracts of L.album, L.purpureum, and L.maculatum at concentrations of 0.25–0.5 μM did not exhibit a cytotoxic effect: dermal fibroblast viability remained at the control level, dense monolayer formation was not impaired, and the proliferation index remained unchanged. The immunophenotype of the cells matched that of the control cultures. At the concentration of 0.5 μM, a decrease in type III collagen production and an increase in fibronectin synthesis were observed (for L.purpureum and L.maculatum). Reduction in the severity of stress-induced cellular senescence were observed in all studied groups. Increasing the extract concentration to 1.5 μM was accompanied by a decrease in viability and morphological signs of inhibited proliferation.
Conclusions. Phytoextracts of Lamium album, Lamium purpureum and Lamium maculatum exert a dose-dependent effect on human dermal fibroblasts in vitro. Concentrations up to 0.5 μM are maximally tolerated. These data demonstrate the cellular safety of Lamium plant extracts in this concentration range and a potential antifibrotic effect when the concentration is increased to 1.5 μM.
Keywords: human dermal fibroblasts, Lamium album, Lamium purpureum, Lamium maculatum.
The work was carried out within the framework of task 3.2.1 “To identify the nature of the action of total phytoextracts, individual hydroxycinnamic acids and flavonoids on human skin fibroblasts in vitro” (No. GR 20230395) of the subprogram “Chemical foundations of life processes” (Bioorganic chemistry) of the State scientific research program “Chemical processes, reagents and technologies, bioregulators and bioorganic chemistry”.
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Submitted 22.01.2026
Accepted 19.06.2026
Information about authors:
Victoryia A. Tsiarletskaya – postgraduate student, assistant of the Chair of Pharmaceutical Chemistry with the course of the Faculty for Advanced Training & Retraining, Belarusian State Medical University, https://orcid.org/0009-0000-8848-4617, email: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.;
R.I. Lukashou – Candidate of Pharmaceutical Sciences, associate professor, head the Chair of Pharmaceutical Chemistry with the course of the Faculty for Advanced Training & Retraining, Belarusian State Medical University, https://orcid.org/0000-0001-5234-6319
A.V. Butenko – junior researcher, Laboratory of Molecular Biology and Cell Biotechnology, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus;
Z.B. Kvacheva – Candidate of Biological Sciences, leading researcher, Laboratory of Molecular Biology and Cell Biotechnology, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus;
A.G. Poleshko – Candidate of Biological Sciences, head of the Laboratory of Molecular Biology and Cell Biotechnology, Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus.
