DOI: https://doi.org/10.22263/2312-4156.2024.3.56
A.S. Bakun, N.S. Gurina
Study of the antibacterial and antifungal activity of the Galega officinalis L. herb
Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2024;23(3):56-62.
Abstract.
Objectives. To study the antibacterial and antifungal activity of extracts from the herb Galega officinalis L.
Material and methods. The object of the study was various extracts from the herb Galega officinalis L.: decoction of Galega officinalis L. (State Pharmacopoeia of the Republic of Belarus, Volume 1, 2nd edition «Infusions, decoctions, teas»), as well as aqueous-alcoholic extracts (30%, 50%, 70%), evaporated and diluted with purified water. Purified water served as the control for all samples. The antibacterial activity was studied in vitro (diffusion method from agar wells) on the following three cultures of microorganisms: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923). The antifungal effect was studied using test strains of Candida albicans (ATCC 10231). The presence of antimicrobial activity was determined by measuring the diameters of the growth inhibition of test bacteria (light zone around the well) of the studied extracts and the control. The results were processed using the STATISTICA 10 program.
Results. The studied samples of Galega officinalis L. showed the greatest activity against Pseudomonas aeruginosa: a decoction of the herb Galega officinalis L. and a 70% alcohol extract inhibited the growth of the bacterium by more than 20 mm compared to the control (25.5±1.65 mm and 25.5±1.66 mm, respectively), a 50% alcohol extract – by 18.25±1.18 mm, a 30% extract – by 10.00±0.71 mm. The growth of E. Coli was inhibited by a 70% alcoholic extract from the herb Galega officinalis L. (9.5±1.04 mm). A decoction of Galega officinalis L. displayed its activity against Staphylococcus aureus (13.25±1.37 mm), a decoction and a 30% alcoholic extract showed antifungal action (14.75±1.79 and 11.00±0.57 mm).
Conclusions. Thus, the antibacterial and antifungal activity of extracts from the herb Galega officinalis L. has been proved.
Keywords: Galega officinalis L., antimicrobial activity, agar diffusion method, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans.
Acknowledgments. The authors express their gratitude to the head of the Laboratory of Nosocomial Infections of the educational establishment “Belarusian State Medical University”, Candidate of Biological Sciences Zh.F. Tsirkunova for the provided support when conducting research.
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Submitted 15.05.2024
Accepted 14.06.2024
Information about authors:
A.S. Bakun – postgraduate of the Chair of Organization of Pharmacy, Belarusian State Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anastasiya S. Bakun;
N.S. Gurina – Doctor of Biological Sciences, professor, dean of the pharmaceutical faculty, Belarusian State Medical University.
