Yorshik O.A., Buzuk G.N.
Antiviral activity of proanthocyanidins of Comarum palustre L. and Thuja occidentalis L.
Education Establishment "Vitebsk State Order of Peoples’ Friendship Medical University", Republic of Belarus
Abstract.
The conditions for assessing the viability of bovine kidney cells using the test with 3,4,5-dimethylthiazole-2-ilo-2,5-diphenyltetrazole (MTT) have been optimized. The antiviral activity of proanthocyanidins of Comarum palustre L., Thuja occidentalis L. and the derived and purified amounts of proanthocyanidins has been investigated. Proanthocyanidins have been found to possess the antiviral activity in vitro according to preventive and curative scheme. The positive effect of proanthocyanidins of the rhizomes and roots of Comarum palustre L. and cones of Thuja occidentalis L. has been determined in the test of MTT cells recovery which indicates the cellular mechanism of the antiviral effect expressed in the strengthening of cells resistance to the cytopathic effect of the virus. Thus, the main group of biologically active substances due to which the rhizomes with roots of Comarum palustre L. and cones of Thuja occidentalis L. possess antiviral activity are proanthocyanidins.
Under the influence of extracts of the rhizomes and roots of Comarum palustre L., proanthocyanidins and cones of Thuja occidentalis L. on the cells in the zone of the low and high dilutions of the virus the statistically significant (P<0,05) increase of the optical density in comparison with the control was observed, which indicates their antiviral activity according to the «prophylactic» scheme.
In the zone of low dilutions of the virus the statistically significant (P<0,05) increase of the optical density is observed under the influence of extracts of the rhizomes and roots of Comarum palustre L., proanthocyanidins and cones of Thuja occidentalis L. which indicates their antiviral activity according to the «therapeutic» scheme.
Key words: Comarum palustre L., Thuja occidentalis L., proanthocyanidins, MTT, antiviral activity.
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