Matusevich E.A., Kosinets V.A.
Metabolic correction of intrahepatic inflammatory response in experimental generalized purulent peritonitis
Education Establishment "Vitebsk State Order of Peoples’ Friendship Medical University", Republic of Belarus
Abstract.
Objectives. To study the dynamics of expression of MAC387 - a marker of the inflammatory response in the liver in experimental generalized purulent peritonitis and the possibility of its correction with drugs of the metabolic type of action.
Material and methods. Experiment was conducted on 55 rabbits of the chinchilla breed. Generalized purulent peritonitis was modelled by the introduction into the abdominal cavity of the aerobic and anaerobic mixture of E.coli and B.fragilis. In 6 hours after the infection surgical treatment was carried out and in the postoperative period during 5 days the preparations Citoflavin or Neoton were used. The animals were taken from the experiment and the material (liver sections) was extracted in 6 hours after the initiation of peritonitis and also on the 1st, 3rd and 5th day after the surgery. After fixing and standard histological wiring serial sections were prepared. Immunohistochemical staining was performed using antibodies to macrophages Anti-Macrophage antibody MAC387 (ab49408, «Abcam»). Image analysis was made with the use of a computer system with a digital camera and licensed software by means of which the level of expression of immunohistochemical marker MAC387 (ab49408) was evaluated.
Results. MAC387-positive macrophages are detected in the liver both in the norm and in experimental purulent peritonitis, in peritonitis the expression level of MAС387 marker of the intrahepatic inflammation increasing significantly. This mechanism may be a predictor of further development of destructive processes in the liver with the disturbance of its function, as well as systemic inflammation and multiple organ failure. The preparation containing succinic acid Citoflavin, in comparison with the creatine phosphate drug Neoton, more effectively reduces the level of expression of MAС387+ cells in the liver.
Conclusion. Citoflavin application in the postoperative period of experimental generalized purulent peritonitis enables the correction of the intensity of the intrahepatic inflammatory cell-mediated response, reduces the degree of the liver tissue alteration and exerts the cytoprotective effect.
Key words: experimental generalized purulent peritonitis, MAС387+ macrophages, citoflavin, neoton, correction, intrahepatic inflammatory response.
References
1. Sartelli M, Catena F, Ansaloni L, Moore E, Malangoni M, Velmahos G, Coimbra R, Koike K, Leppaniemi A, Biffl W, Balogh Z, Bendinelli C, Gupta S, Kluger Y, Agresta F, Di Saverio S, Tugnoli G, Jovine E, Ordonez C, Gomes CA, Junior GA, Yuan KC, Bala M, Peev MP, Cui Y, Marwah S, Zachariah S, Sakakushev B, Kong V, Ahmed A, Abbas A, Gonsaga RA, Guercioni G, Vettoretto N, Poiasina E, Ben-Ishay O, Díaz-Nieto R, Massalou D, Skrovina M, Gerych I, Augustin G, Kenig J, Khokha V, Tranà C, Kok KY, Mefire AC, Lee JG, Hong SK, Segovia Lohse HA, Ghnnam W, Verni A, Lohsiriwat V, Siribumrungwong B, Tavares A, Baiocchi G, Das K, Jarry J, Zida M, Sato N, Murata K, Shoko T, Irahara T, Hamedelneel AO, Naidoo N, Adesunkanmi AR, Kobe Y, Attri A, Sharma R, Coccolini F, El Zalabany T, Khalifa KA, Sanjuan J, Barnabé R, Ishii W. Complicated intra-abdominal infections in a worldwide context: an observational prospective study (CIAOW Study). World J Emerg Surg. 2013 Jan 3;8(1):1.
2. Gürlich R, Adámková V, Ulrych J, Balík M, Ferko A, Havel E, Jabor A, Janík V, Kala Z, Klementa I, Kolář M, Krška Z, Kysela P, Lischke R, Neoral C, Vyhnánek F, Zajak J, Zbořil P, Třeška V. Basic principles of diagnosis and treatment of secondary peritonitis - recommendations of experts with the support of SIS. Rozhl Chir. 2014 Jun;93(6):334-48, 350-2.
3. Efimenko NA, Rozanov VE, Bolotnikov AI. Immunopatogenez i kontseptsiia sovremennoi immunoterapii peritonita u postradavshikh s tiazheloi sochetannoi travmoi zhivota [Immunopathogenesis and modern concept of immunotherapy of peritonitis in patients with severe combined trauma of the abdomen]. Moscow. RF: Avtograf, 2008. 302 p.
4. Yamamoto K, Ohmoto M, Matsumoto S, Nagano T, Kobashi H, Okamoto R, Tsuji T. Activated liver macrophages in human liver diseases. J Gastroenterol Hepatol. 1995;10 Suppl 1:72-6.
5. Tomita M, Yamamoto K, Kobashi H, Ohmoto M, Tsuji T. Immunohistochemical phenotyping of liver macrophages in normal and diseased human liver. Hepatology. 1994 Aug;20(2):317-25.
6. McGuinness PH, Painter D, Davies S, McCaughan GW. Increases in intrahepatic CD68 positive cells, MAC387 positive cells, and proinflammatory cytokines (particularly interleukin 18) in chronic hepatitis C infection. Gut. 2000 Feb;46(2):260-9.
7. DeLeo MJ, Gounis MJ, Hong B, Ford JC, Wakhloo AK, Bogdanov AA Jr. Carotid artery brain aneurysm model: in vivo molecular enzyme-specific MR imaging of active inflammation in a pilot study. Radiology. 2009 Sep;252(3):696-703.
8. Gutierrez M, Forster FI, McConnell SA, Cassidy JP, Pollock JM, Bryson DG. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques / M. Gutierrez [et al.]. Vet Immunol Immunopathol. 1999 Nov;71(3-4):321-34.
9. Burwitz BJ, Reed JS, Hammond KB, Ohme MA, Planer SL, Legasse AW, Ericsen AJ, Richter Y, Golomb G, Sacha JB. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol. 2014 Sep;96(3):491-501.
