Karpuk I.Y.
The importance of matrix metalloproteinases-8 and -9 in the development of oral mucosa allergopathology caused by intolerance to tooth prosthetic materials
Education Establishment "Vitebsk State Order of Peoples’ Friendship Medical University", Republic of Belarus
Abstract.
Objectives. To determine the role of metalloproteinases system in the pathogenesis of an allergy to dentoprosthetic materials for broadening the notion about pathogenetic mechanisms of its formation with the purpose of the elaboration of biochemical criteria to assess the risk of the development of an allergy to dentoprosthetic materials.
Material and methods. We have established the content of matrix metalloproteinases (MMP)-8 and -9 in the oral fluid in 40 patients allergic to dentoprosthetic materials.
Results. The results of the comparative immunofermental research of the content of matrix metalloproteinases -8 and -9 in the oral fluid of people with various constructional materials of teeth and tooth alignments restorations are presented.
Proteolysis system including matrix metalloproteinases family is characterized by the increase in the activity of MMP-8 and -9 in the saliva of patients with intolerance to dentoprosthetic materials 33,3 mg/ml and 37,3 mg/ml respectively (in the control group – 9 mg/ml and 14 mg/ml).
It has been revealed that MMP-8 and-9 in the oral fluid may serve as a marker of an allergy to dentoprosthetic materials, this being proved by the correlation analysis of the obtained data between the results of the reaction of allergen-induced damage of leukocytes (RADL) and MMP-8 and MMP-9 levels. The following dependences have been found: 1) direct strong correlation of RADL (ZnCl2 0,01%) and MMP-8 levels (R=0,76; p=0,001) and MMP-9 (R=0,8; p=0,001); 2) direct moderate correlation of RADL (CrCl3 0,01%) and MMP-8 levels (R=0,49; p=0,0019) and MMP-9 (R=0,34; p=0,04); 3) direct moderate correlation of RADL (NiCl2 0,01%) and MMP-8 levels (R=0,52; p=0,001) and MMP-9 (R=0,57; p=0,001); 4) direct moderate correlation of RADL (CoCl2 0,005%) and MMP-8 levels (R=0,53; p=0,005) and MMP-9 (R=0,36; p=0,02).
Conclusion. Studying qualitative and quantitative characteristics of MMP and their inhibitors in the oral fluid is a new direction of scientific researches which will enable the elaboration of new approaches to the diagnosing, prognostication and scientific substantiation of the choice of the most informative criteria to assess individual predisposition to the development of an allergy to dentoprosthetic materials.
Key words: oral fluid, allergy, matrix metalloproteinases.
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