Lukashov R.I., Moiseyev D.V.
Phenolic compounds of Rudbeckia hirta flowers and their immunotropic activity
Education Establishment "Vitebsk State Order of Peoples’ Friendship Medical University", Republic of Belarus
Abstract.
The presence of ten flavonoids and four hydroxycinnamonic acids has been confirmed in Rudbeckia hirta flowers harvested in Belarus by means of high performance liquid chromatography with mass spectrometric detection. Rudbeckia hirta flowers dried by air shadow method at room temperature contain the highest quantity of biologically active substances. Thermal drying (40, 60, 80 and 100°C) with forced ventilation reduces the content of biologically active substances. The optimal mode of drying Rudbeckia hirta flowers may be considered air shadow one at room temperature. Within two years of storage in a dark place at room temperature Rudbeckia hirta flowers are stable. Indices of authenticity and purity during two years of storage are practically unchanged. Immunotropic effect of a tincture and an infusion in phythogemagglutinin induced lymphocyte blast transformation reaction is shown. Patuletrin and caffeic acid practically do not show any immunotropic action in response induced by PHA blast transformation lymphocytes reaction.
Key words: phenolic compounds, flowers, Rudbeckia hirta, drying, storage, immunotropic activity.
References
1. Novikov DK, Novikov PD, Titova ND. Immunokorrektsiia, immunoprofilaktika, immunoreabilitatsiia [Immunocorrection, immunoprevention, immunorehabilitation]. Vitebsk, RB: VGMU; 2006. 198 р.
2. Kattsung GB. Bazisnaia i klinicheskaia farmakologiia [Basic and clinical pharmacology]: v 2 t. 2-e izd., pererab. i dop. Moscow, RF: BINOM; Saint-Petersburg, RF: Dialekt; 2008. T. 2. 784 р.
Sokolov SIa. Fitoterapiia i fitofarmakologiia [Phytotherapy and phytopharmacology]: ruk. dlia vrachei. Moscow, RF: Meditsinskoe informatsionnoe agentstvo; 2000. 976 р.
4. Kukes VG, red, Starodubtsev AK, red. Klinicheskaia farmakologiia i farmakoterapiia [Clinical pharmacology and pharmacotherapy]: ucheb. dlia vuzov. 2-e izd., ispr. Moscow, RF: GEOTAR-Media; 2006. 640 р.
5. Lukashov RI, Moiseev DV. Kolichestvennoe opredelenie flavonoidov i gidroksikorichnykh kislot v tsvetkakh rudbekii shershavoi [Quantification of flavonoids and hydroxycinnamic acids in flowers rudbeckia rough]. Retsept. 2013;(5):95-105.
6. Rudbekii shershavoi tsvetki [Rudbeckia flowers rough]. Novosti ekspertizy i registratsii. 2014;(5):26-30.
7. Lukashov RI, Moiseev DV. Immunotropnaia aktivnost' tsvetkov rudbekii shershavoi (Rudbeckia hirta L.) [Immunotropic Activity flowers rudbeckia rough (Rudbeckia hirta L.)]. Retsept. 2013;(4):96-103.
8. TKP 431-2012 (02041), BY. Proizvodstvo lekarstvennykh sredstv. Ispytaniia stabil'nosti = Vytvorchasts' lekavykh srodkaў. Vyprabavanni stabil'nastsi [Production of medicines. Stability tests]. Vved. 29.11.12. Minsk, RB: Departament farmatsevticheskoi promyshlennosti; 2012. 66 р.
9. Lukashov RI. Obosnovanie srokov godnosti tsvetkov rudbekii shershavoi [Justification of the shelf life of flowers rudbeckia rough]. V: Molodaia farmatsiia – potentsial budushchego: sb. materialov IV Vseros. nauch. konf. studentov i aspirantov s mezhdunar. uchastiem, Sankt-Peterburg, 14-15 apr. 2014 g. Saint-Petersburg, RF; 2014. р. 489-492.
10.Sheriakov AA, red. Gosudarstvennaia farmakopeia Respubliki Belarus' (GF RB II) [State pharmacopeia of Republic of Belarus]: v 2 t. Molodechno, RB: Pobeda; 2012. T. 1: Obshchie metody kontrolia kachestva lekarstvennykh sredstv. 1217 р.
