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Katlinskaya Y.V.*, Krastelyova I.M.**, Ustinovich Y.A.**, Shilkina E.V.**, Shishko G.A.**, Voytenok N.N.***
The soluble form of interleukin-8 receptor CXCR2 as a marker of an inflammation in perinatal pathology
*State Establishment «Republican Scientific-Practical Center of Transfusiology and Medical Biotechnologies», Minsk, Republic of Belarus   
**State Educational Establishment «Belarusian Medical Academy of Post-Graduate Education», Minsk, Republic of Belarus
***Fund of molecular humatology and immunology, Moscow, Russian Federation

Vestnik VGMU. 2015;14(6):70-77.

Objectives. To determine the levels of interleukin-8 soluble receptor CXCR2 (sCXCR2) as a marker of neutrophilic leukocytes involvement in the inflammatory process during the pathology of pregnancy and respiratory distress syndrome (RDS) in premature infants.
Material and methods. Soluble sCXCR2 level in the supernatants of activated neutrophils and human biological fluids was determined by «sandwich» enzyme-linked immunoassay.
Results. Soluble CXCR2 was found in the amniotic fluid in the second trimester in the pathology of pregnancy. The levels of sCXCR2 directly correlated with the levels of soluble tumor necrosis factor y (TNF- ) receptor p55, which can indicate that sCXCR2 is produced by neutrophils in response to the increasing concentration of TNF-) caused by infection. We have also examined the groups of preterm infants with RDS devoid of infectious pathology and with RDS associated with pneumonia, intrauterine infection and sepsis. It has been shown that the levels of sCXCR2 in the urine of newborns on the 1st day after birth were significantly reduced in patients with RDS and infection in comparison with the group of infants with RDS without accompanying infectious pathology.
Conclusions. Thus the levels of sCXCR2 may reflect the severity of inflammatory changes during RDS, and serve as a signal for an early treatment. Moreover, the determination of sCXCR2 in newborns urine is not associated with conducting invasive procedures.
Key words:  systemic inflammatory response, infection, amniotic fluid, urine, soluble CXCR2.


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