DOI: https://doi.org/10.22263/2312-4156.2016.3.47
Usychenko E.N., Bazhora Y.I., Usychenko E.M.
Genetic profiles of patients with chronic hepatitis C and B
Odessa National Medical University, Odessa, Ukraine
Vestnik VGMU. 2016;15(3):47-52.
Abstract.
Objectives. To conduct a comparative analysis of the frequency of polymorphisms of IL-4 (C589T), IL-10 (G1082A), TNFa (G308A) in patients with chronic hepatitis B (CHB) and chronic hepatitis C (CHC), residing in Odessa region for the improvement of the diagnosis quality on the basis of the obtained genetic criteria.
Material and methods. Cytokine gene polymorphism was studied using amplification of the corresponding regions of the genome by PCR method. The structure of the primers used and the parameters of temperature cycles were employed from the international genomic data base.
Results. The differences in allele frequencies of TNFR gene (G308A) (p<0,05) were revealed in ethnically homogeneous groups of the inhabitants of Odessa region among patients with chronic hepatitis B and chronic hepatitis C. The comparative analysis of allelic polymorphism of IL-4 (C589T) and IL-10 (G1082A) in patients with CHC and CHB didn’t show any statistically significant difference between them.
Conclusions. Significant differences in gene polymorphism of TNFC (G308A) in patients with CHC and CHB indicate the possibility of genetic profile individualization in different hepatites. Thus, the absence of significant difference in the frequency of genotypes of IL-4 (C589T) and IL-10 (G1082A) can serve as a confirmation of the important role of these cytokines in the immunological genetic profile segment of patients.
Key words: chronic hepatitis В, chronic hepatitis С, gene polymorphism of cytokines.
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Information about authors:
Usychenko E.N. - Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases of Odessa National Medical University, Ukraine;
Bazhora Y.I. – Doctor of Medical Sciences, professor, head of the Chair of Clinical Immunology, Genetics & Medical Biology of Odessa National Medical University, Ukraine;
Usychenko E.M. – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases of Odessa National Medical University, Ukraine.
Correspondence address: Ukraine, 65062, Odessa, 67A Fontanskaya doroga str.,160. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Usychenko E.N.
