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Usychenko E.N., Usychenko E.M., Bazhora Y.I.
Immune status and gene polymorphism of cytokines IL-10, IL-4, TNF in patients with chronic hepatitis C depending on the degree of fibrosis
Odessa National Medical University, Odessa, Ukraine

Vestnik VGMU. 2017;16(1):50-58.

Objectives. To study gene polymorphisms of IL-10 (G1082A), IL-4 (C589T) and TNF  (G308A) and cellular immunity indices (CD3+, CD4+, CD8+, CD16+, CD19+) in patients with chronic hepatitis C (CHC) and healthy persons belonging to the ethnically homogeneous group of the Odessa region residents, as well as to identify possible association of certain genotypes and the degree of liver fibrosis.
Material and methods. The study included 100 patients with CHC. The control group consisted of 30 people having no diseases of the hepatobiliary system. Traditional diagnostic methods stipulated by the protocol in effect were used. Polymorphism was studied using  amplification of the corresponding regions of the genome by PCR method. The structure of the primers used was described in the data base GenBank. The evaluation of morphological changes in the liver (the degree of activity and the severity of fibrotic changes) was made according to METAVIR scale using a noninvasive method Fibrotest.
Results. A certain level of statistical significance of certain genotypes of cytokines, cellular immunity indices and the degree of liver fibrosis was established. Patients with the homozygous CC genotype IL-4 had smaller fibrotic changes in the liver compared to the carriers of the homozygous TT genotype (p<0,01).
Patients with the homozygous genotype GG TNFPPhad a lower degree of liver fibrosis in comparison with the carriers of the homozygous genotype AA (p<0,01). Patients with smaller changes in cellular immunity indices (reduction of CD3+, CD4+, CD16+, CD19+) had a lower degree of liver fibrosis (p<0,01). Patients with higher levels of CD8 + had a higher degree of liver fibrosis (p<0,01).
Conclusions. The presence of an interconnection between the degree of liver fibrosis and certain genotypes of IL-4 and TNF  allows to use the received information as a criterion of the progression speed of fibrotic processes in the liver. The expressiveness of the changes in cellular immunity indices is an additional criterion of the degree of morphological disturbances in the liver tissue.
Key words: chronic hepatitis C, gene polymorphism, liver fibrosis, cell-mediated immunity.


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Information about authors:

Usychenko E.N. – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases, Odessa National Medical University;
Usychenko E.M. – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases, Odessa National Medical University;
Bazhora Y.I. – Doctor of Medical Sciences, professor, head of the Chair of Clinical Immunology, Genetics & Medical Biology, Odessa National Medical University.

Correspondence address: Ukraine, 65062, Odessa, 67A Fontanskaya doroga str.,160. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Ekaterina N. Usychenko.

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