DOI: https://doi.org/10.22263/2312-4156.2017.2.58
Belyaeva L.E., Fedchenko A.N., Lazuko S.S., Ligetskaya I.V., Orekhova N.I.
The peculiarities of disorders of NO-dependent mechanisms regulating coronary vessels tone of rats which felt the stressors effect in the prenatal period
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2017;16(2):58-69.
Abstract.
Objectives. To investigate the role of nitric oxide produced by the endotheliocytic and inducible NO-synthase in the mechanisms of decreasing coronary vessels tone and disorders of the myocardium contractility in mature rats whose mothers felt the stressors effect during their pregnancy.
Material and methods. The experiments were performed on the isolated by Langendorff method hearts (n=121) of white mongrel 3-month rats whose mothers were exposed to chronic stress during their pregnancy. The hearts contracting in isometrical mode in the conditions of the constant coronary flow, were perfused with standard Krebs-Henseleit solution a) on intact NO-synthase activity; b) on adding a non-selective inhibitor of NO-synthase of N-ω-nitro-L-arginine methyl ester (L-NAME, 60 mcM) to the solution; c) on adding selective inhibitor of inducible NO-synthase of S-methylisothiourea (S-MT, 10 mcM) to the perfused solution. Changes in coronary perfusion pressure (CPP), developed intraventricular pressure (DIP) and the velocity of contraction and relaxation of the myocardium during inhibition of different isoforms of NO-synthases were investigated.
Results. Under the influence of both L-NAME and S-MT decreased values of CPP increased statistically significantly in the hearts of males that underwent prenatal stress. Despite the fact that there was no reduction of CPP in the isolated hearts of females after prenatal stress, the applied inhibitors of NO-synthase did not cause any statistically significant increase of CPP in the isolated hearts of females whose mothers were exposed to the stressors effect during pregnancy. Moreover, the values of CPP registered in the hearts of the females of the group “the offspring-stress” after the introduction of L-NAME into the perfusion solution turned out to be by 16,6-27,8 % lower than those in the isolated hearts of the control female offspring after using this NO-synthase inhibitor. Neither L-NAME nor S-MT restored reduced after prenatal stress DIP, as well as the velocity of contraction and relaxation of the myocardium.
Conclusions. The character of nitric oxide formation by the endotheliocytic and inducible NO-synthase is significantly different in the coronary vessels of mature females and males whose mothers underwent chronic stress during pregnancy. Hyperproduction of nitric oxide is an important cause of the reduction of basal vascular tone of the isolated hearts of 3-month males whose mothers experienced the stressors effect during pregnancy. The excessive formation of NO in the myocardium of rats exposed to prenatal stress, is not the main cause of decreased contractile function of the heart in these animals.
Key words: prenatal stress, coronary vessels, nitric oxide, endotheliocytic NO-synthase, inducible NO-synthase.
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Information about authors:
Belyaeva L.E. – Candidate of Medical Sciences, associate professor, head of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Fedchenko A.N. – Master of Medical Sciences, teacher of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Lazuko S.S. – Candidate of Biological Sciences, associate professor, head of the Chair of Normal Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Ligetskaya I.V. – Master of Biological Sciences, teacher of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Orekhova N.I. – MSc (Medicine) student of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210023, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Pathologic Physiology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Lyudmila E. Belyaeva.
