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Myadelets O.D.1, Lebedeva E.I.1, Myadelets N.Y.2
Phosphatase-positive stem cells of rat skin while its posttraumatic regeneration in different conditions of wound inflicting
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Vitebsk State Medical College, Vitebsk, Republic of Belarus

Vestnik VGMU. 2018;17(3):44-57.

When injured the skin of the mammals regenerates with scar formation. Organotypic regeneration with complete restoration of the original structure of the skin was successful in not numerous experimental conditions. The mechanisms of the formation of organotypic regenerates have not been studied.
Objectives. To study with the help of the enzyme of alkaline phosphomonoesterase (AP) the stem cells of the skin (SC) and the nature of their changes in the organotypic healing of skin wounds inflicted with prolonged deep hypothermia.
Material and methods. For this investigation, 114 mature white rats of both sexes were used, whose wounds were inflicted in the state of normothermia and at the depth of hypothermia. To achieve general deep hypothermia, after anesthesia with ethyl ether, the animals were cooled with a «water-ice» mixture up to the rectal temperature of +18°C. Further in the interscapular area, full-thickness stamped wounds with a diameter of 1 cm were inflicted, and the hypothermia condition was prolonged by 6 hours. Then the animals were warmed at a speed of 1°C per 4-5 minutes. Control animals were wounded similarly on normothermia. The skin of the interscapular region in intact rats and the regenerate with surrounding skin in experimental animals were taken immediately after cooling and at various times after warming, poured into paraffin, the sections were stained with hematoxylin-eosin. Cryostat sections were prepared from the frozen in liquid nitrogen material, in which AP was detected by the Burstone method.
Results and conclusions. The skin wounding against the background of prolonged deep hypothermia leads to the formation of the skin type regenerate with the restoration of hair and sebaceous glands. In the epithelization of the wound surface and the subsequent formation of the skin derivatives, the leading role is played by the SC epithelium of thickening (bulge) of the hair follicles of the skin surrounding the wound. There is an increase in the amount and activity of AP in the SC of the bulge, these cells extend towards the interfollicular epidermis. It starts to reveal AP-positive cells, which give an over-all sharply positive reaction to the enzyme. Around the hair follicles of the skin surrounding the wound, an AP-positive extracellular matrix appears. The alkaline phosphomonoesterase contained in it can obviously contribute to the organotypic regeneration of the skin.
Key words: skin, wounds, hypothermia, AP-positive stem cells.


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Information about authors:
Myadelets O.D. – Doctor of Medical Sciences, professor, head of the Chair of Histology, Cytology & Embryology, Vitebsk State Order of Peoples’ Friendship Medical University;
Lebedeva E.I. – Candidate of Biological Sciences, associate professor of the Chair of Histology, Cytology & Embryology, Vitebsk State Order of Peoples’ Friendship Medical University;
Myadelets N.Y. – lecturer of histology and genetics, Vitebsk State Medical College.

Correspondence address: Republic of Belarus, 210023, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Histology, Cytology & Embryology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Elena I. Lebedeva.

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