Yarotskaya N.N., Kosinets V.A., Karalkova N.K.
Changes in the functional activity of the liver in experimental generalized purulent peritonitis with the application of energotropic correction
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2018;17(6):46-54.
Objectives. To estimate the effect of energotropic drugs on the functional activity of the liver in experimental generalized purulent peritonitis.
Material and methods. The experiment was performed on 59 rabbits. Purulent peritonitis was modelled by means of intra-abdominal administration of an aerobic-anaerobic mixture of E. coli and B. fragilis. In 6 hours after the initiation of peritonitis, surgical treatment was performed with the use of metabolic drugs Cytoflavin containing succinic acid, nicotinamide, Riboxin and Riboflavin or Neotone containing creatine phosphate during 5 days in the postoperative period. The animals were taken out of the experiment in 6 hours after the initiation of peritonitis, as well as on the 1st, 3rd, 5th day after the operation. Functional activity was assessed by the indices of the intensity of free-radical oxidation, enzyme activity, and carbohydrate metabolism products in liver homogenates.
Results. The obtained data testified to the development of the liver metabolic disorders in experimental generalized purulent peritonitis, as evidenced by the change in pro/antioxidant balance, the level of indicator, excretory, lysosomal enzymes and their substrates. The use of drugs possessing energotropic action allowed to reduce the negative effect of bacterial toxins in the postoperative period, exerted on the functional state of the liver in comparison with the control group of animals that did not receive any energotropic support.
Conclusions. The initiation of experimental purulent peritonitis already in 6 hours leads to the development of metabolic disorders in the liver with an increase in free radical oxidation processes and inhibition of antioxidant system factors, which indicates the damage to the hepatocytes membrane structures. The use of the energotropic action preparations in the postoperative period contributes to an increase of the antioxidant system power, which is accompanied by a decrease in the severity of cytolytic and destructive processes in the liver parenchyma.
Key words: experimental generalized purulent peritonitis, rabbits, liver, energotropic drugs.
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Information about authors:
Yarotskaya N.N. – research officer of the Scientific-Research Laboratory, Vitebsk State Order of Peoples’ Friendship Medical University,
Kosinets V.A. – Doctor of Medical Sciences, professor of the Chair of Hospital Surgery with the courses of Urology & Pediatric surgery, Vitebsk State Order of Peoples’ Friendship Medical University,
Karalkova N.K. – Candidate of Medical Sciences, associate professor, acting head of the Chair of Ophthalmology, Vitebsk State Order of Peoples’ Friendship Medical University,