Sheibak V.M., Pauliukavets A.Y., Doroshenko E.M., Olekhnovich E.A.
Acute effect of single introduction of taurine: specific or nonspecific?
Grodno State Medical University, Grodno, Republic of Belarus
Vestnik VGMU. 2019;18(2):37-43.
Objectives. To reveal the dynamic changes in the amino acid pool of the plasma after a single injection of a pharmacological dose of taurine.
Material and methods. The experiment was performed on 29 female rats weighing 120-140 g, with free access of animals to food and water. Taurine was administered to rats intragastrically at a dose of 500 mg / kg of body weight. Animals were decapitated in 15, 30, and 90 min after taurine administration. The blood plasma was used for analysis. Determination of free amino acids was carried out by the method of reversed-phase HPLC.
Results. A single intragastric administration of taurine (500 mg/kg) leads to an increase in the concentration of this amino acid in the blood plasma, the maximum level of which was recorded after 30 min (837.5±45.89 μmol / l, whereas in the control it was 142.0±18.95 μmol/l), however, the most pronounced changes in the amino acid pool of the blood plasma were observed in 90 minutes after taurine administration. A single administration of taurine reduced the total amount of amino acids and their nitrogen-containing metabolites in the blood plasma.
Conclusions. Thus, a decrease in the total amount of amino acids and their nitrogen-containing derivatives in rats’ blood plasma, caused by the administration of taurine, is likely to indicate a nonspecific stimulation of protein synthesis.
Obviously, the osmoregulatory, antioxidant and hormonal effects of taurine will be affected to the greatest extent by the concentration-dependent changes of amino acids in the blood plasma and extracellular fluid, while long-term administration of it in small (close to physiological) doses largely implies a more subtle effect on the signal / regulatory mechanisms.
Key words: taurine, free amino acids, blood plasma, rats, nitrogen-containing metabolites of amino acids.
1. Sheybak VM, Sheybak LN. Biological role of taurine in mammals. Med Novosti. 2005;(10):15-8. (In Russ.)
2. Sheybak VM, Sheybak LN. Biosynthesis and taurine metabolism. Zhurn GrGMU. 2005;(1):9-12. (In Russ.)
3. Schaffer S, Kim W. Effects and Mechanisms of Taurine as a Therapeutic Agent. Biomol Ther (Seoul). 2018 May;26(3):225-241. doi: http://dx.doi.org/10.4062/biomolther.2017.251
4. Wang J, Qi C, Liu L, Zhao L, Cui W, Tian Y, et al. Taurine Protects Primary Neonatal Cardiomyocytes Against Apoptosis Induced by Hydrogen Peroxide. Int Heart J. 2018 Jan;59(1):190-196. doi: http://dx.doi.org/10.1536/ihj.16-372
5. Wu H, Jin Y, Wei J, Jin H, Sha D, Wu JY. Mode of action of taurine as a neuroprotector. Brain Res. 2005 Mar 21;1038(2):123-31.
6. Das J, Sil PC. Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways and apoptosis in rats. Amino Acids. 2012 Oct;43(4):1509-23.
7. Marcinkiewicz J, Kontny E. Taurine and inflammatory diseases. Amino Acids. 2014 Jan;46(1):7-20. doi: http://dx.doi.org/10.1007/s00726-012-1361-4
8. Schaffer SW, Shimada-Takaura K, Jong CJ, Ito T, Takahashi K. Impaired energy metabolism of the taurine-deficient heart. Amino Acids. 2016 Feb;48(2):549-58. doi: http://dx.doi.org/10.1007/s00726-015-2110-2
9. Katakawa M, Fukuda N, Tsunemi A, Mori M, Maruyama T, Matsumoto T, et al. Taurine and magnesium supplementation enhances the function of endothelial progenitor cells through antioxidation in healthy men and spontaneously hypertensive rats. Hypertens Res. 2016 Dec;39(12):848-856. doi: http://dx.doi.org/10.1038/hr.2016.86
10. L’Amoreaux WJ, Marsillo A, Idrissi A. Pharmacological characterization of GABAA receptors in taurine-fed mice. J Biomed Sci. 2010;17( Suppl 1):S14. doi: http://dx.doi.org/10.1186/1423-0127-17-S1-S14
11. Chan CY, Sun HS, Shah SM, Agovic MS, Ho I, Friedman E, et al. Direct interaction of taurine with the NMDA glutamate receptor subtype via multiple mechanisms. Adv Exp Med Biol. 2013;775:45-52. doi: http://dx.doi.org/10.1007/978-1-4614-6130-2_4
12. Ito T, Miyazaki N, Schaffer S, Azuma J. Potential antiaging role of taurine via proper protein folding: a study from taurine transporter knockout mouse. Adv Exp Med Biol. 2015;803:481-7. doi: http://dx.doi.org/10.1007/978-3-319-15126-7_38
13. Ghosh S, Chowdhury S, Das AK, Sil PC. Taurine ameliorates oxidative stress induced inflammation and ER stress mediated testicular damage in STZ-induced diabetic Wistar rats. Food Chem Toxicol. 2019 Feb;124:64-80. doi: http://dx.doi.org/10.1016/j.fct.2018.11.055
14. Shetewy A, Shimada-Takaura K, Warner D, Jong CJ, Mehdi AB, Alexeyev M, et al. Mitochondrial defects associated with B-alanine toxicity: relevance to hyper-beta-alaninemia. Mol Cell Biochem. 2016 May;416(1-2):11-22. doi: http://dx.doi.org/10.1007/s11010-016-2688-z
15. Ulrich-Merzenich G, Zeitler H, Vetter H, Bhonde RR. Protective effects of taurine on endothelial cells impaired by high glucose and oxidized low density lipoproteins. Eur J Nutr. 2007 Dec;46(8):431-8
Information about authors:
Sheibak V.M. – Doctor of Medical Sciences, professor of the Chair of Biologic Chemistry, Grodno State Medical University;
Pauliukavets A.Y. – Candidate of Biological Sciences, associate professor of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, Grodno State Medical University;
Doroshenko E.M. – Candidate of Biological Sciences, associate professor, leading research officer of the Scientific-Research Laboratory, Grodno State Medical University;
Olekhnovich E.A. – the fifth-year medical student, Grodno State Medical University.