Goncharova A.I., Okulich V.K., Ziamko V.Y., Senkovich S.A.
Antimicrobial activity of lysozyme as a nonspecific resistance factor
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2019;18(4):40-45.
Objectives. To study the modern etiological structure of sialadenitis with PCR diagnosis taken into account; to establish the role of lysozyme in the development of inflammatory diseases of the large salivary glands.
Material and methods. 86 patients with sialadenitis were examined. These patients underwent inpatient treatment in the Vitebsk Regional Clinical Hospital. In parallel with the bacteriological method of the study, multiplex PCR diagnosis was performed in real time. To detect DNA a set of reagents «Septoscreen» («Litech», Russia) was used. The result was evaluated by means of the program Bio Rad CFX Manager 3.0.
Results. During bacteriological studies, 70 isolates were isolated in 67 (77.9%) patients with sialadenitis, and inoculation negative results were obtained in 19 cases (22.1%). The modern etiological structure of sialadenitis was studied taking into consideration multiplex PCR diagnosis. Multiplex PCR diagnosis in comparison with the bacteriological method is by 16.6% more sensitive. During the experiment the antimicrobial activity of lysozyme against standard strains and isolates isolated from patients with inflammatory diseases of the large salivary glands was determined.
Conclusions. The modern etiological structure of sialadenitis has been studied with PCR diagnosis taken into account, the role of lysozyme in the development of inflammatory diseases of the large salivary glands has been established.
Key words: sialadenitis, inflammatory disease of the large salivary glands, PCR diagnosis, lysozyme.
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Information about authors:
Goncharova A.I. – senior lecturer of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Okulich V.K. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University,
Ziamko V.Y. – postgraduate of the Chair of Anesthesiology & Critical Care Medicine with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Senkovich S.A. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University.