Pauliukevich A.N., Belyaeva L.Eu.
The system of nitric oxide production in rats which have undergone prenatal stress
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(2):35-43.
Objectives. To assess the level of nitric oxide (NO) production, intensity of the lipid peroxidation (LPO) processes in the blood serum and the low grade inflammation level as well as to measure the blood pressure (BP) in rats whose mothers were exposed to the impact of stressors during pregnancy.
Material and methods. Outbred pregnant rats (180-220 g) were devided into 2 groups, 10 animals in each. Pregnant rats out of «stress» group underwent the influence of different types of stress factors: fasting during the day, contact with cats’ excrements during the day and 20 minute immobilization in water (t=23±2); while pregnancy in the second group of rats developed in normal conditions («control»). Serum concentration of nitrates / nitrites (NO3-/NO2-), endothelial and inducible isoforms of NO-synthase (eNOS and iNOS, respectively), asymmetric dimethylarginine (ADMA), diene conjugates (DC), malondialdehyde (MDA), C-reactive protein (hsCRP) and BP were determined in 3-month-old offspring.
Results. Stressors exposure in the prenatal period led to the increase in blood pressure, iNOS, hsCRP and MDA concentration without any statistically significant changes of the level of NO3-/NO2- in the blood serum of both male and female rats. In male rats (but not females), whose mothers were exposed to stress during pregnancy, the decrease in the level of eNOS and the increase in the content of ADMA and DC in the blood serum were revealed.
Conclusions. Disorders of the NO synthesis system in prenatally stressed rats have sexual features. In 3-month offspring of rats, which were exposed to the impact of stressors during pregnancy, lipid peroxidation intensity and blood pressure were increased and systemic low-grade inflammation developed.
Key words: prenatal stress, nitric oxide, NO-synthase, asymmetric dimethylarginine, lipid peroxidation, systemic low grade inflammation, arterial blood pressure.
The sources of financing: funds allocated within the frames of the theme task of State Research Programs (GPNI) of the Republic of Belarus «To evaluate the delayed sequel of the prenatal stress to the coronary vessels tension and to substantiate the methods for revealed disturbances prevention».
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Information about authors:
Pauliukevich A.N. – Master of Medical Sciences, senior lecturer of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University;
Belyaeva L.E. – Candidate of Medical Sciences, associate professor, head of the Chair of Pathologic Physiology, Vitebsk State Order of Peoples’ Friendship Medical University.