DOI: https://doi.org/10.22263/2312-4156.2020.3.96
Zybleva S.V., Zyblev S.L.
The peculiarities of dendritic cells subpopulations in patients on kidney transplantation
Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus
Vestnik VGMU. 2020;19(3):96-105.
Abstract.
Objectives. To study the dynamics of dendritic cells subpopulations in patients after kidney transplantation in the early postoperative period.
Material and methods. We have examined 197 patients who underwent kidney transplantation. All patients were divided into 3 groups. The first group (PGF, n=101) consisted of patients with satisfactory primary graft function without rejection episodes, the second group (PGD, n=82) included patients with primary graft dysfunction without rejection episodes, the third group (GR, n=14) was composed of patients with primary graft dysfunction and graft rejection verified on the basis of renal transplant biopsy. A comparison group (CG) consisted of 90 healthy volunteers. Graft function was assessed according to the following criteria: primary graft function was characterized by creatinine level below 300 μmol/l on the 7th day after surgery; when creatinine concentration was equal to or higher than 300 μmol/l and (or), in case of need for dialysis during the first week after transplantation, the state was classified as kidney graft dysfunction. Immunological examination of patients was carried out before the transplantation, and on the 1st, the 3rd and the 7th days after the transplantation. The levels of LIN-HLA-DR+CD11c+CD123- (myeloid DC) and LIN-HLA-DR+CD11c-CD123+ (plasmacytoid DC) were determined.
Results. We have noted an increase in the absolute and relative indicator of the total number of DC, as well as absolute values of the number of myeloid and plasmacytoid DCs in the group of recipients with PGF from the third to the seventh day of the post-transplantation period. While in the GR group, a decrease in the level of these cells beginning with the third post-transplantation day was observed.
Conclusions. The assessment of the myeloid and plasmacytoid dendritic cells level in kidney transplant recipients widens the possibilities of laboratory monitoring in the early post-transplantation period with the purpose of timely adjustment of the provided immunosuppressive therapy.
Key words: LIN-HLA-DR+CD11c+CD123-, LIN-HLA-DR+CD11c-CD123+, kidney transplantation.
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Information about authors:
Zybleva S.V. – Candidate of Medical Sciences, academic secretary, immunologist, Republican Research Center for Radiation Medicine and Human Ecology,
ORCID: https://orcid.org/0000-0003-3061-5324
Zyblev S.L. – Candidate of Medical Sciences, associate professor, surgeon of the surgical department (transplantation, reconstructive and endocrine surgery), Republican Research Center for Radiation Medicine and Human Ecology,
ORCID: https://orcid.org/0000-0002-0968-6630
Correspondence address: Republic of Belarus, 246000, Gomel, 5 Ilicha str., Republican Research Center for Radiation Medicine and Human Ecology, Scientific Department. E-mail: zyb-svetlana@ yandex.by – Svetlana V. Zybleva.
