DOI: https://doi.org/10.22263/2312-4156.2020.4.14
Pashinskaya E.S.1, Pabiarzhyn V.V.1, Semenov V.M.2, Konevalova N.Y.1, Sushko G.G.2
The comparative analysis of the effect of unicellular and multicellular parasites on the change in the expression of markers in rat glioma tissue in an experiment
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Vitebsk State University named after P.M. Masherov, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(4):14-21.
Abstract.
The goal is to conduct a comparative analysis of the effect of parasites on the change in the expression of markers in rat glioma tissue by the example of Ascaris and Toxoplasma invasion in an experiment, depending on the observation period.
It has been revealed that A. suum invasion in the dose of 40 eggs per 1 g of animal body weight 2.16 times increases GFAP expression in the biopsy specimens of the tumor tissue of rat C6 glioma in situ by the 7th day of infestation development; the expression of S 100 by the 7th - 14th day – 2.05-2.53 times; the Ki 67 index by the 7th - 14th day – 2.22-2.28 times.
Infection of rats in the dose of 10,000 Toxoplasma tachysoites leads to 1.79-2.34 times increase in GFAP expression compared to the control series at all stages of observation periods. Compared to series 2 GFAP expression increases 1.87-3.42 times from the 14th to the 28th day.
1.45-3.99 times increase in S 100 expression is detected at all stages of observation period compared to the control. Comparison with the second series has shown that the expression of S 100 increased 1.87 5.91 times.
Toxoplasma invasion leads to 3.18 6.15 times increase in Ki-67 compared to the control. The proliferative activity index in comparison with the results obtained in the second series of the experiment by the 2st and the 28th day is significantly 6.29 8.43 times higher.
Key words: rat, glioma, immunohistochemical markers, GFAP, S 100, Ki-67, ascariasis, toxoplasmosis.
References
1. Dolbin DA, Lutfullin MKh. Prevalence of asacariasis in humans, age and demographic dynamics. Uchen Zap Kaz Gos Akad Veterinar Meditsiny im NE Baumana. 2015;222(2):83-5. (In Russ.)
2. Toigombaeva VS. Intestinal parasitic diseases of the population of Batken region of the Republic of Kyrgyzstan. Med Parazitologiia Parazitar Bolezni. 2009;(2):31-3. (In Russ.)
3. Smith PJ, Theis B, McCartney S, Brown M. Helminths: an unrecognised disease burden prevalent among migrants in the gastroenterology clinic. Frontline Gastroenterol. 2011 Apr;2(2):124-129. doi: http://dx.doi.org/10.1136/fg.2010.003392
4. Ershova IB, Mochalova AA, Lokhmatova IA, Manashova MG, Petrenko OV. Nonspecific manifestations of helminthiasis in children. Zdorov'e Rebenka. 2015;(8):48-50. (In Russ.)
5. Mikhailova NR, Kalinina TN, Tuchkov DIu, Losin EI, Abakumov GG. Toxoplasmosis of the brain in patients with HIV infection in the city of Orenburg. Vestn Orenburg Gos Un-ta. 2015;(1):138-44. (In Russ.)
6. Barycheva LIu, Golubeva MV, Kabulova MA, Kostornaia IV. Clinical and morphological features of developmental defects in children with congenital cytomegalovirus and toxoplasma infections. Ros Vestn Perinatologii Pediatrii. 2015;(3):50-7. (In Russ.)
7. Ngô HM, Zhou Y, Lorenzi H, Wang K, Kim T-K, Zhou Y, et al. Toxoplasma modulates signature pathways of human epilepsy Neurodegeneration & Cancer. Sci Rep. 2017 Sep;7(1):11496. doi: http://dx.doi.org/10.1038/s41598-017-10675-6
8. Ermolenko AE. Etiological classification of tumors and mechanisms of carcinogenesis. Mat Morfologiia Elektron Mat Med-biol Zhurn. 2012;11(2). Rezhim dostupa: http://sgma.alpha-design.ru/MMORPH/N-34-html/ermolenko/ermolenko.htm. Data dostupa: 04.09.2020. (In Russ.)
9. Anichkov NM. The doctrine of apoptosis at the present stage. Uchen Zap SPbGMU im akad IP Pavlova. 1999;6(4):31-40. (In Russ.)
10. Kolotov KA, Mashkovtcev OV, Bein BN. Immunohistochemical features of glial brain tumors. Med Al'm. 2012;(4):66-9. (In Russ.)
11. Nadyrov EA, Rogov IuI, Dubrovskii ACh, Voropaev EV, Achinovich SL, Krylov AIu, i dr. Immunohistochemical methods for studying neoplasms of various genesis: instruktsiia po primeneniiu № 160-1110: utv M-vom zdravookhraneniia Resp Belarus' 11.02.2011 g. Gomel, RB; 2011. 20 р. (In Russ.)
12. Bekish VIa. Method for obtaining invasive ascaris eggs. V: Piatyi Respublikanskii s"ezd spetsialistov klinicheskoi laboratornoi diagnostiki Respubliki Belarusi: materialy s"ezda. Minsk, RB; 1997. Р. 140-1. (In Russ.)
13. Pashinskaia ES, Pobiarzhin VV, Egorov SK, Kosova MS. Toxoplazma gondii cultivation technique in vivo. V: Shchastnyi AT, red. Studencheskaia meditsinskaia nauka KhKhI veka. III Forum molodezhnykh nauchnykh obshchestv: materialy XVIII mezhdunar nauch-prakt kon studentov i molodykh uchenykh i III Foruma molodezh nauch o-v (Vitebsk, 14-15 noiab 2018 g.). V 2 ch. Ch 2. Vitebsk, RB; 2018. Р. 597-9. (In Russ.)
14. Sapozhnikov AG, Dorosevich AE. Histological and microscopic technique. Smolensk, RF: SAU; 2000. 479 р. (In Russ.)
15. Honeycutt J, Hammam O, Fu C-L, Hsieh MH. Controversies and challenges in research on urogenital schistosomiasis-associated bladder cancer. Trends Parasitol. 2014 Jul;30(7):324-32. doi: http://dx.doi.org/10.1016/j.pt.2014.05.004
16. Ploeg M, Aben KКH, Kiemeney LA. The present and future burden of urinary bladder cancer in the world. World J Urol. 2009 Jun;27(3):289-93. doi: http://dx.doi.org/10.1007/s00345-009-0383-3
17. Salem HK, Mahfouz S. Changing patterns (age, incidence, and pathologic types) of schistosoma-associated bladder cancer in Egypt in the past decade. Urology. 2012 Feb;79(2):379-83. doi: http://dx.doi.org/10.1016/j.urology.2011.08.072
18. Buisson Y. Control of Opisthorchis viverrini infection for cholangiocarcinoma prevention. Bull Soc Pathol Exot. 2017 Feb;110(1):61-67. doi: http://dx.doi.org/10.1007/s13149-017-0544-8
19. Smout MJ, Sripa B, Laha T, Mulvenna J, Gasser RB, Young ND, et al. Infection with the carcinogenic human liver fluke, Opisthorchis viverrini. Mol Biosyst. 2011 May;7(5):1367-75. doi: http://dx.doi.org/10.1039/c0mb00295j
20. Bekish O-IaL, Bekish VIa. Mutagenic effect of metabolites of migrating ascaris larvae (Ascaris suum). Ves Nats Akad Navuk Belarusi Ser Biial Navuk. 2000;(2):109-13. (In Russ.)
21. Zorina VV, Bekish O-IaL, Bekish VIa. Impact of migrating roundworm larvae on the host genome during pregnancy. Vestn VGMU. 2009;8(2):120-7. (In Russ.)
22. Stibel VV, Danko NN, Svarchevskii OA. Effect of intravital secretions of nematodes on the genome of white rats. Teoriia Praktika Parazitar Boleznei Zhivotnykh. 2010;(11):463-6. (In Russ.)
23. Blaszkowska J. Disturbance of mouse pregnancy after injection of Ascaris homogenate during early organogenesis. Wiad Parazytol. 2000;46(3):369-78.
Information about authors:
Pashinskaya Е.S. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor, dean of the Overseas Students Training Faculty, Vitebsk State Order of Peoples’ Friendship Medical University;
Semenov V.M. – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
Konevalova N.Y. – Doctor of Biological Sciences, professor, pro-rector for academic affairs, Vitebsk State Order of Peoples’ Friendship Medical University;
Sushko G.G. – Doctor of Biological Sciences, associate professor, head of the Chair of Ecology & Nature Conservation, Vitebsk State University named after P.M. Masherov.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Еkaterina S. Pashinskaya.
