Kosova M.S.1, Pashinskaya E.S.1, Semenov V.M.1, Konevalova N.Y.1, Sushko G.G.2
Embryotoxic effect of Toxoplasma gondii depending on the dose and duration of the parasite development in an experiment
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Vitebsk State University named after P.M. Masherov, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(5):80-86.
The goal is to study the embryotoxic effect of Toxoplasma gondii, depending on the dose and duration of the invasion during infection of female rats after pregnancy.
It has been revealed that at a dose of infection of 25 tachyzoites, the number of living embryos on the 7th day of Toxoplasma development was 1.2-1.5 times less than the control (p˂0.04). The number of dead embryos on the 14th and the 21st days exceeded 1.2-6.0 times (p˂0.05) the control values.
The level of resorptions on the 7th day and the 14th day after infection exceeded the control 2.7 times (p˂0.03) and 1.1 times (p˂0.01) and on the 21st day – 3.3 times (p˂0.008). The analysis of the number of resorptions revealed that by the 14th day after infection, their number was 2.4 times lower than on the 7th day (p ˂0.02) and by the 21st day it exceeded the data obtained by the 7th day 1.2 times (p˂0.04) and by the 14th day – 3 times (p˂0.004).
In female rats infected with a dose of 50 tachyzoites, the number of living embryos on the 7th day was 1.6 times less than in the control group (p˂0.003), and also than in animals infected with a dose of 25 tachyzoites per 1 g of body weight – 1.3 times (p˂0.002). By the 14th and the 21st days, the number of living embryos differed from the indicators of intact animals being 1.6-1.9 times lower, and from the indicators of females of the 4th, the 5th, the 6th groups being 1.2-1.3 times (p˂0.002) lower.
On the 14th and the 21st days, the number of dead embryos exceeded the control 2.4-7.7 times (p˂0.05). The analysis of the data in comparison with the animals infected with a lower dose revealed 1.28-2.4 times (p˂0.04) increase in the number of dead embryos.
In females infected at a dose of 50 tachyzoites on the 7th day the level of resorptions exceeded the control values 3.3 times (p˂0.004) and on the 14th day and the 21st day – 1.7-2.3 times (p˂0.05).
The number of resorptions was higher than that of animals infected at a dose of 25 tachyzoites by the 7th day – 1.2 times (p˂0.05), by the 14th day – 1.5 times (p˂0.04), by the 21st day – 1.4 times (p˂0.003).
Key words: rats, Toxoplasma gondii, embryotoxic effect.
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Information about authors:
Kosova M.S. – laboratory assistant of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Pashinskaya Е.S. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Semenov V.M. – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Konevalova N.Y. – Doctor of Biological Sciences, professor, pro-rector for academic affairs, Vitebsk State Order of Peoples’ Friendship Medical University;
Sushko G.G. – Doctor of Biological Sciences, associate professor, head of the Chair of Ecology & Nature Conservation, Vitebsk State University named after P.M. Masherov.