Zybleva S.V., Zyblev S.L.
Indicators of CD3-CD16+CD56+ and CD3+CD16+CD56+ lymphocytes with satisfactory primary kidney graft function
Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus
Vestnik VGMU. 2020;19(6):122-131.
Objectives. To study the dynamics of CD3-CD16+CD56+ and CD3+CD16+CD56+ lymphocytes in patients after kidney transplantation with satisfactory primary graft function.
Material and methods. 197 recipients underwent kidney transplantation. All patients were divided into 3 groups. The first group (PGF, n=101) consisted of patients with satisfactory primary graft function without rejection episodes, the second group (PGD, n=82) included patients with primary graft dysfunction without rejection episodes, the third group (GR, n=14) was composed of patients with primary graft dysfunction and graft rejection verified on the basis of renal transplant biopsy. A comparison group (CG) consisted of 90 healthy volunteers. Graft function was assessed according to the following criteria: primary graft function was characterized by creatinine level below 300 μmol/l on the 7th day after surgery; when creatinine concentration was equal to or higher than 300 μmol/l and (or) in case of need for dialysis during the first week after transplantation, the state was classified as kidney graft dysfunction. Immunological examination was carried out on the 1st, the 3rd, the 7th, the 30th and the 90th days after the transplantation. The level of CD3-CD16+CD56+ and CD3+CD16+CD56+ lymphocytes was determined.
Results. In the PGF group, a significant predominance of the absolute NK level from the 3rd day to the 30th day was revealed only in comparison with the GR group. The relative NK level was significantly higher in the PGF group compared to the GR group beginning with the 30th day. No correlation was found between the relative and absolute NK levels and creatinine levels throughout the study. There was a significant increase in the absolute and relative NKT level beginning with the 30th day in the PGF group compared to the PGD and GR groups. A negative correlation was found between the absolute NKT level and creatinine level on the 30th and the 90th days and the relative level of these lymphocytes with the creatinine level on the 90th day.
Conclusions. The results of the study can be used for the immunological monitoring in the early post-transplantation period.
Key words: CD3-CD16+CD56+ and CD3+CD16+CD56+, kidney transplantation.
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Information about authors:
Zybleva S.V. – Candidate of Medical Sciences, academic secretary, immunologist, Republican Research Center for Radiation Medicine and Human Ecology;
Zyblev S.L. – Candidate of Medical Sciences, associate professor, surgeon of the surgical department (transplantation, reconstructive and endocrine surgery), Republican Research Center for Radiation Medicine and Human Ecology,