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Aliakhnovich N.S., Velichynskaya V.G., Yanchanka U.V.
The effect of intracutaneous аutoserumhistaminotherapy on the lymphocytes phenotype and the concentration  of immunoglobulins in patients with chronic urticaria
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus

Vestnik VGMU. 2020;19(6):70-78.

Objectives. To assess the effect of аutoserumhistaminotherapy on the blood low affinity IgE receptor (CD23; FcᶓRII), CD19 (B-lymphocytes main marker), CD40 (B-lymphocytes activation marker) expression on lymphocytes and immunoglobulins A, M, G, E levels in patients with chronic urticaria.
Material and methods. The study involved 20 patients (5 – with cold urticaria and 15 – with chronic unspecified urticaria) and 13 healthy volunteers. The expression of CD23, CD19, CD40 and A, M, G, E serum immunoglobulins levels were determined, as well as the change in these parameters under the influence of а аutoserumhistaminotherapy. The blood serum for treatment was taken during the exacerbation of the disease or after the provocation, histamine dihydrochloride was added and when the rash disappeared was injected intradermally, alternating points starting from 0.1 to 0.4 ml for 10 days (Instructions for use No. 047-0614 of the Ministry of Health of the Republic of Belarus).
Results. The groups of patients with an increase or decrease of CD23+-lymphocytes were distinguished. Autoserum with histamine normalized the increased level of CD19+CD40+-lymphocytes (p=0.017) in patients with chronic urticaria, decreased the level of CD23+-lymphocytes in cold urticaria (p=0.043), reduced the number of CD19+CD40+-lymphocytes in the group with the increased expression of CD23 on lymphocytes in unspecified urticaria. In patients with chronic urticaria, the level of immunoglobulin E 6.7-7 times exceeded the norm. In case of unspecified urticaria, IgG exceeded the norm by 22.7% and remained elevated by 33.8% after аutoserumhistaminotherapy. The concentration of IgA in the blood in case of cold urticaria after аutoserumhistaminotherapy increased significantly (p<0.05).
Conclusions. Autoserumhistaminotherapy had a positive effect on the phenotype of blood lymphocytes, reducing the increased number of CD19+CD40+-lymphocytes in chronic urticaria and the number of CD23+-lymphocytes in cold urticaria. A significant increase in the blood serum of IgE concentration was found in patients with chronic urticaria, a moderate increase of IgG was revealed in patients with unspecified urticaria. Under the influence of аutoserumhistaminotherapy, the concentration of immunoglobulin A increased.
Key words: CD19, CD23, CD19, CD40, chronic urticaria, immunoglobulins A, M, G, E.


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Information about authors:
Aliakhnovich N.S. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
Velichynskaya V.G. – Candidate of Medical Sciences, associate professor of the General Practitioner Chair with the course of Outpatient Therapy, Vitebsk State Order of Peoples’ Friendship Medical University,
Yanchanka U.V. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Natalia S. Aliakhnovich.

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