DOI: https://doi.org/10.22263/2312-4156.2020.6.70
Aliakhnovich N.S., Velichynskaya V.G., Yanchanka U.V.
The effect of intracutaneous аutoserumhistaminotherapy on the lymphocytes phenotype and the concentration of immunoglobulins in patients with chronic urticaria
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(6):70-78.
Abstract.
Objectives. To assess the effect of аutoserumhistaminotherapy on the blood low affinity IgE receptor (CD23; FcᶓRII), CD19 (B-lymphocytes main marker), CD40 (B-lymphocytes activation marker) expression on lymphocytes and immunoglobulins A, M, G, E levels in patients with chronic urticaria.
Material and methods. The study involved 20 patients (5 – with cold urticaria and 15 – with chronic unspecified urticaria) and 13 healthy volunteers. The expression of CD23, CD19, CD40 and A, M, G, E serum immunoglobulins levels were determined, as well as the change in these parameters under the influence of а аutoserumhistaminotherapy. The blood serum for treatment was taken during the exacerbation of the disease or after the provocation, histamine dihydrochloride was added and when the rash disappeared was injected intradermally, alternating points starting from 0.1 to 0.4 ml for 10 days (Instructions for use No. 047-0614 of the Ministry of Health of the Republic of Belarus).
Results. The groups of patients with an increase or decrease of CD23+-lymphocytes were distinguished. Autoserum with histamine normalized the increased level of CD19+CD40+-lymphocytes (p=0.017) in patients with chronic urticaria, decreased the level of CD23+-lymphocytes in cold urticaria (p=0.043), reduced the number of CD19+CD40+-lymphocytes in the group with the increased expression of CD23 on lymphocytes in unspecified urticaria. In patients with chronic urticaria, the level of immunoglobulin E 6.7-7 times exceeded the norm. In case of unspecified urticaria, IgG exceeded the norm by 22.7% and remained elevated by 33.8% after аutoserumhistaminotherapy. The concentration of IgA in the blood in case of cold urticaria after аutoserumhistaminotherapy increased significantly (p<0.05).
Conclusions. Autoserumhistaminotherapy had a positive effect on the phenotype of blood lymphocytes, reducing the increased number of CD19+CD40+-lymphocytes in chronic urticaria and the number of CD23+-lymphocytes in cold urticaria. A significant increase in the blood serum of IgE concentration was found in patients with chronic urticaria, a moderate increase of IgG was revealed in patients with unspecified urticaria. Under the influence of аutoserumhistaminotherapy, the concentration of immunoglobulin A increased.
Key words: CD19, CD23, CD19, CD40, chronic urticaria, immunoglobulins A, M, G, E.
References
1. Novikov DK, Novikov PD, Titova ND. Clinical Immunology and Allergology: ucheb dlia studentov uchrezhdenii vyssh obrazovaniia po spetsial'nostiam «Lechebnoe delo», «Pediatriia». Minsk, RB: Vysh shk; 2019. 495 р. (In Russ.)
2. Zuberbier T, Aberer W, Asero R, Abdul Latiff AH, Baker D, Ballmer-Weber B, et al. The EAACI/GAІLEN/EDF/WAO guideline for the definition, classification, diagnosis and management of urticaria. Allergy. 2018 Jul;73(7):1393-1414. doi: http://dx.doi.org/10.1111/all.13397
3. Kolkhir P, Church MK, Weller K, Metz M, Schmetzer O, Maurer M. Autoimmune chronic spontaneous urticarial. J Allergy Clin Immunol. 2018 Mar;141(3):1165-6.
4. Burton OT, Oettgen HC. Beyond immediate hypersensitivity: evolving roles for IgE antibodies in immune homeostasis and allergic diseases. Immunol Rev. 2011 Jul;242(1):128-43. doi: http://dx.doi.org/10.1111/j.1600-065X.2011.01024.x
5. Aliakhnovich NS, Ianchenko VV, Asirian EG, Martinovich VP, Golubovich VP. Detection of IgE-positive blood leukocytes with synthetic peptide and monoclonal antibodies. Immunopatologiia Allergologiia Infektologiia. 2016;(2):28-34. (In Russ.)
6. Matthews AL, Koo CZ, Szyroka J, Harrison N, Kanhere A, Tomlinson MG. Regulation of Leukocytes by TspanC8 Tetraspanins and the «Molecular Scissor» ADAM10. Front Immunol. 2018 Jul 2;9:1451. doi: http://dx.doi.org/10.3389/fimmu.2018.01451
7. Gould HJ, Sutton BJ. IgE in allergy and asthma today. Nat Rev Immunol. 2008 Mar;8(3):205-17. doi: http://dx.doi.org/10.1038/nri2273
8. Siracusa MC, Kim BS, Spergel JM, Artis D. Basophils and allergic inflammation. J Allergy Clin Immunol. 2013 Oct;132(4):789-801. doi: http://dx.doi.org/10.1016/j.jaci.2013.07.046.
9. Semenova IV, Vykhristenko LR, Novikov DK. Clinical efficacy of intradermal autoserum therapy in patients with pollen allergy. Immunopatologiia Allergologiia Infektologiia. 2014;(1):33-41. (In Russ.)
10. Minina ES, Novikov PD. Decrease in the level of IgE antibodies after intradermal autoserotherapy for atopic bronchial asthma in children. Immunopatologiia Allergologiia Infektologiia. 2015;(4):44-52. (In Russ.)
11. Patil S, Sharma N, Godse K. Autologous serum therapy in chronic urticaria. Indian J Dermatol. 2013 May;58(3):225-6. doi: http://dx.doi.org/10.4103/0019-5154.110833
12. Novikov DK, Novikov PD, Velichinskaia OG, Semenova IV, Ishchenko OV, Titova ND. Intradermal autohistamine therapy is a highly effective method of treating allergic diseases, which reduces the level of IgE antibodies. Allergologiia Immunologiia. 2017;18(1):25-8. (In Russ.)
13. Gushchin IS, Ilina NI, red. Urticaria and angioedema: rekomendatsii dlia prakt vrachei. Moskva, RF: Farmarus Print Media; 2007. 128 р. (In Russ.
14. Novikov DK, Velichinskaia OG. Evaluation of the efficacy and safety of intradermal autohistamine therapy in chronic spontaneous urticaria. Immunopatologiia Allergologiia Infektologiia. 2014;(4):70-8. (In Russ.)
15. Kmiecik T, Otocka-Kmiecik A, Górska-Ciebiada M, Ciebiada M. T lymphocytes as a target of histamine action. Arch Med Sci. 2012 Feb;8(1):154-61. doi: http://dx.doi.org/10.5114/aoms.2012.27295
16. Zwadlo-Klarwasser G, Braam U, Mühl-Zürbes P, Schmutzler W. Macrophages and lymphocytes: alternative sources of histamine. Agents Actions. 1994 Jun;41:C99-100. doi: http://dx.doi.org/10.1007/BF02007785
17. Gloudemans AK, Lambrecht BN, Smits HH. Potential of immunoglobulin A to prevent allergic asthma. Clin Dev Immunol. 2013;2013:542091. doi: http://dx.doi.org/10.1155/2013/542091
Information about authors:
Aliakhnovich N.S. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-9533-8914
Velichynskaya V.G. – Candidate of Medical Sciences, associate professor of the General Practitioner Chair with the course of Outpatient Therapy, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-7501-2351
Yanchanka U.V. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-9355-8534
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Natalia S. Aliakhnovich.
