The content of sex steroids and their reception in experimental animals under the conditions of chronic radiation impact
Vitebsk State Awarded the «Badge of Honour» Order Veterinary Medicine Academy, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(6):86-91.
The problem of the possible consequences of long-term low doses exposure to ionizing radiation still remains little-studied. The complexity associated with assessment and prognostication in these studies are linked with the activity of cell reparation systems that help the organisms to adapt to the changing internal and external environmental conditions.
Objectives. To show the current state of the problem of small doses impact of ionizing radiation, the main focus being on the content in the blood and reception of estradiol and progesterone in a target organ (rat’s uterus) exposed to external radiation.
Material and methods. The experiments were conducted on mongrel laboratory female rats. Standard diagnostic kits were used to measure the blood levels of estradiol and progesterone. The reception of the steroids was studied with the help of a radioligand method.
Results. The early and remote effects of long-time ionizing radiation on the content of estradiol and progesterone in the blood and their reception in target organs have been studied. It has been established that in the conditions of an experiment external gamma radiation didn’t cause any reliable change in sex steroids of the blood. A change in the quantity of estradiol and progesterone cytosolic receptors has been found in the uterus of irradiated female rats.
Conclusions. Chronic gamma radiation exerts a destabilizing effect on the system of steroid regulation, which can lead to a disturbance of physiological functions in the body.
Key words: estradiol, progesterone, blood, steroid hormone content, hormone receptors, γ-irradiation.
1. Vasilenko IIa, Vasilenko OI. Chernobyl Accident: Medical Consequences of Low Dose Irradiation. Biul Atom Energii. 2003;(4):48-52. (In Russ.)
2. Popov EG, Naumov AD. Radiation-induced changes in the characteristics of androgen-specific proteins and their contribution to hormonal dysregulation. Solodkov AP, Chirkin AA, red. V: Sovremennye problemy biokhimii: ucheb posobie dlia studentov i magistrantov vyssh ucheb zavedenii po biol spetsial'nostiam. Vitebsk, RB: UO «VGU im PM Masherova»; 2010. Gl 10. Р. 231-54. (In Russ.)
3. Popov EG. Androgens, androgen-specific proteins, and ionizing radiation: monografiia. Minsk, RB: Pravo i ekonomika; 2013. 221 р. (In Russ.)
4. Iarmonenko SP, Vaison AA. Radiobiology of humans and animals: ucheb dlia biol spetsial'nostei vuzov. 3-e izd, pererab i dop. Moscow, RF: Vyssh shk; 2004. 549 р. (In Russ.)
5. Bekman IN. Radioecology and ecological radiochemistry: uchebnik dlia vuzov. 2-e izd, ispr i dop. Moscow, RF: Iurait; 2019. 497 р. (In Russ.)
6. Dedov VI, Dedov II, Stepanenko VF. Radiation endocrinology. Moscow, RF: Meditsina; 1993. 208 р. (In Russ.)
7. Grebeniuk AN, Strelova OIu, Legeza VI, Stepanova EN. Fundamentals of Radiobiology and Radiation Medicine: ucheb posobie. Saint Petersburg, RF: Foliant; 2012. 232 р. (In Russ.)
8. Dedov II, Melnichenko GA, Fadeeva VF. Endocrinology: uchebnik. Moscow, RF: GEORTAR-Media; 2014. 432 р. (In Russ.)
9. Popov EG, Seliavko VV. Influence of radioecological factors on the indices of androgen-dependent regulation of the organism in irradiated parents and their offspring. Ekol Vestn. 2009;(3-4):59-68. (In Russ.)
10. Panin LE. Transport forms of steroid hormones in the blood, their relationship with the development of some physiological and pathological processes. Biul SO RAMN. 2012;32(1):31-7. (In Russ.)
11. Filchenkov GN, Popoff EH, Naumov AD. The low dose gamma ionising radiation impact upon cooperativity of androgen-specific proteins. J Environ Radioact. 2014 Jan;127:182-90. doi: http://dx.doi.org/10.1016/j.jenvrad.2013.02.002
12. Mel'nik SN. Effect of bimethyl on the specific binding of estradiol in rat cardiomyocytes under conditions of ionizing radiation and stress. Problemy Zdorov'ia Ekologii. 2010;(2):103-6. (In Russ.)
Information about authors:
Navumau A.D. – Doctor of Biological Sciences, professor of the Chair of Radiology & Biophysics, Vitebsk State Awarded the «Badge of Honour» Order Veterinary Medicine Academy.