DOI: https://doi.org/10.22263/2312-4156.2020.6.99
Pabiarzhyn V.V.
Changes of gene expression indices in rat tissues during reproduction of experimental C6 glioma in combination with trichinosis
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2020;19(6):99-104.
Abstract.
Objectives. To study changes of gene expression indices in rat tissues during reproduction of experimental C6 glioma in combination with trichinosis.
Material and methods. The experiment was conducted on 90 female Wistar rats weighing 180-200 g. In female rats of the first («control with tumor») and the second groups («glioma combined with trichinosis», the infection at a dose of 10 Trichinella spiralis larvae per 1 gram of the animal body weight ) C6 glioma tumor was simulated in situ.
In animals of the first group of the experiment, material was taken on the 14th, the 21st, the 28th, the 35th days of tumor development, respectively (tumor, the liver, the lungs, the brain), and in females of the second group – on the 7th (the 14th day of tumor development), the 14th (the 21st day of tumor development), the 21st (the 28th day of tumor development), the 28th day after infection (the 35th day of tumor development).
The animals of the third group were healthy (10 head of rats). Tissue biopsies were taken in them once (the liver, the lungs, the brain).
The comparative expression of the studied genes was carried out after normalization of each of the samples to the level of the control genes GAPDH and ACTIN-β with the help of the Real-Time Bio-Rad amplifier. The expression analysis was performed using qbase + and CFX Maestro software.
Results. The infection of animals at a dose of 10 Trichinella spiralis larvae per 1 g of the animal body weight leads to an increase in the expression of survivin (BIRC5), GLI, VEGF and TP53 in the tissues of the lungs, the liver, and the brain compared with the data of the groups «control with tumor» and healthy animals.
Key words: rat, glioma, Trichinella, expression, genes.
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Information about authors:
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor, doctoral candidate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viachaslau V. Pabiarzhyn.
