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Artsiukh T.V., Sokolova T.N., Astrowskaja A.B.
The resistance peculiarities of E.coli and C.albicans clinical isolates forming a biofilm
Grodno State Medical University, Grodno, Republic of Belarus

Vestnik VGMU. 2021;20(1):46-54.

Objectives. To model biofilms of mixed paired cultures of C.albicans and E.coli in vitro and study the characteristics of their sensitivity to antimicrobial drugs (doxycycline, amoxiclav, ofloxacin, fluconazole, clotrimazole) in planktonic form, as well as in the composition of mono- and mixed biofilms.
Material and methods. In the course of the study, the properties and ability to film formation of C.albicans 2924 and E.coli 2646 isolated from mucous membranes in patients with clinical signs of vaginitis were investigated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of antibiotics were determined for planktonic forms of microorganisms: bacteria and candida in the composition of a mono-biofilm, as well as in the composition of a mixed biofilm.
Results. It has been revealed that the studied microorganisms are capable of forming a biofilm. On studying the sensitivity of microorganisms in biofilms (BF) it has been found that the MIC of preparations for mono-biofilms increases 2-4 times, for mixed biofilms 2.5-8.5 times compared to the MIC of preparations for planktonic forms of E.coli and C.albicans.  Bactericidal concentrations of amoxiclav, ofloxacin, doxycycline, fluconazole, clotrimazole for planktonic forms are not effective for biofilm forms of the same microorganisms.
Conclusions. To determine the sensitivity of microorganisms in the composition of biofilms to antibacterial drugs is necessary for the administration of rational antibiotic therapy and the development of empirical antibiotic therapy protocols. It is important to study the mechanisms of bacterial resistance in the composition of biofilms, develop new pharmaceuticals with a high potential for action on biofilms, since standard therapy with high doses of antibiotics (AB) is toxic for the macroorganism, which dictates the need to test new agents.
Key words: resistance, microbial films, antibiotics, antimycotic drugs, minimum inhibitory concentration.

Acknowledgement: The authors express their gratitude to the senior laboratory assistant of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg of Grodno State Medical University Domostoy E.A. for the help provided while conducting investigations.


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Information about authors:
Artsiukh T.V. – lecturer of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, Grodno State Medical University,
Sokolova T.N. – Candidate of Medical Sciences, associate professor of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, Grodno State Medical University,
Astrowskaja A.B. – Candidate of Medical Sciences, associate professor of the Chair of Microbiology, Virology and Immunology named after S.I. Gelberg, senior researcher of morphology group with electron microscopy of the Research Laboratory, Grodno State Medical University,

Correspondence address: Republic of Belarus, 230009, Grodno, 80 Gorky str., Grodno State Medical University, Chair of Microbiology, Virology and Immunology named after S.I. Gelberg. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. –Tatiana V. Artsiukh.

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