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Kolbasova E.A.1, Kiseleva N.I.1, Naumov A.V.2
Sulfur-containing amino acids and their metabolite derivatives in women with climacteric syndrome in post-menopause
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Grodno State Medical University, Grodno, Republic of Belarus

Vestnik VGMU. 2021;20(1):72-80.

Objectives. To study the indicators of the concentrations of sulfur-containing amino acids and their metabolites in the blood serum of women with surgical and natural menopause and to establish correlations with the severity of menopause syndrome, the level of gonadotropic and sex steroid hormones.
Material and methods. We examined 192 women, 93 of them with surgical menopause (group I), 68 with natural menopause (group II), 31 in late reproductive and premenopausal ages (control group). The patients underwent a study of the content of homocysteine (Hcy), cysteine (Cys), glutathione (GSH) and cysteinylglycine (CysGly) in the blood serum by means of high performance liquid chromatography (HPLC).
Results. It has been found that in women with surgical menopause, the median value of homocysteine, cysteine, cysteinylglycine is 1.5 times, by 17.0% and 23.2%, respectively, higher than in women with natural menopause and 2.2, 2.0 and 1.8 times, respectively, higher than in the control group patients (p<0.05). The median concentration of glutathione in the blood in patients with surgical menopause was reduced (by 26.7% (p<0.01)), in patients with natural menopause – by 16.2% lower (p<0.05) than in the control group.
The increase in the severity of the climacteric syndrome (CS) according to the Kupperman-Uvarova scale corresponded to an increase in Hcy, Cys, CysGly and a decrease in GSH in the blood serum of the examined women (p<0.01).
Conclusions. The established direct and inverse statistically significant correlations between indicators of the concentration of sulfur-containing amino acids (their derivatives) and gonadotropic, steroid sex hormones and clinical manifestations of climacteric syndrome testify to the participation of the studied indicators in the development of the menopausal period pathology.
Key words: oophorectomy, surgical menopause, natural menopause, climacteric syndrome, hormones, homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly), glutathione (GSH).


1. Naumov AV. Homocysteine.  Medical and biological problems. Minsk, RB: Prof izd; 2013. 312 р. (In Russ.)
2. Cherviakov AV. Disruption of the molecular asymmetry of amino acids (d / L-enantiomers) in normal aging and neurodegenerative diseases. Asimmetriia. 2010;4(2):77-112. (In Russ.)
3. Garaeva OI. Sulfur Amino Acids as Markers of Stress. Buletinul AŞM Ştiinţele Vieţii Fiziologia şi Sanocreatologia. 2011;(3):50-62. (In Russ.)
4. Ganchar EP, Kazhina MV. The level of free amino acids and their derivatives in blood serum in women of reproductive age with metabolic syndrome. Zhurn GrGMU. 2014;(3):66-70. (In Russ.)
5. Kurtay G, Ozmen B. A comparison of the effects of sequential transdermal versus continuous orally administered hormone replacement therapies on plasma total homocysteine levels in postmenopausal women: a randomized, placebo-controlled study. Eur J Obstet Gynecol Reprod Biol. 2007 Feb;130(2):206-11. doi:
6. Zhang Z, Gu Xiang, Fang X, Tang Z, Guan S, Liu H, et al. Homocysteine and the risk of cardiovascular events and all-cause death in elderly population: a community-based prospective cohort study. Ther Clin Risk Manag. 2020 May;16:471-481. doi:
7. Esse R, Barroso M, Tavares de Almeida I, Castro R. The Contribution of homocysteine metabolism disruption to endothelial dysfunction: state-of-the-art. Int J Mol Sci. 2019 Feb;20(4):867. doi:
8. Kang SC, Sohn EH, Lee SR. Hydrogen Sulfide as a potential Alternative for the Treatment of Myocardial Fibrosis. Oxid Med Cell Longev. 2020 Jan;2020:4105382. doi:
9. Naumov AV, Danilchik IV, Sarana IuV. Three pathways for homocysteine remthylation. Zhurn GrGMU. 2016;(2):27-32. (In Russ.)
10. Yang Q, He GW. Imbalance of homocysteine and H2S: significance, mechanisms, and therapeutic promise in vascular injury. Oxid Med Cell Longev. 2019 May;2019:1-11. doi:
11. Borisenok OA, Bushma MI, Basalai ON, Radkovetc AIu. The biological role of glutathione. Med Novosti. 2019;(7):3-8. (In Russ.)
12. Naumov AV, Doroshenko EM. Determination of homocysteine by HPLC with precolumn derivatization in microvolumes of biological fluids. V: Egorov VV, Gulevich AL, Nazarov VA, otv za vyp. Analitika RB – 2010: sb tez dokl Resp nauch konf po analit khimii s mezhdunar uchastiem «Analitika RB – 2010», Minsk, Belarus’, 14–15 maia 2010 g. Minsk, RB; 2010. Р. 138. (In Russ.)
13. Kolbasova EA, Kiseleva NI, Arestova IM. Comparative clinical and hormonal characteristics of the health status and quality of life of women with surgical and natural menopause. Vestn VGMU. 2014;13(2):78-86. (In Russ.)
14. Perticone M, Maio R, Caroleo B, Sciacqua A, Suraci E, Gigliotti S, et al. Serum γ-glutamyltransferase concentration predicts endothelial dysfunction in naive hypertensive patients. Biomedicines. 2020 Jul;8(7):207. doi:
15. Lee DH, Jacobs DR. Association between serum gamma-glutamyltransferase and C-reactive protein. Atherosclerosis. 2005 Feb;178(2):327-30. doi:
16. Behr GA, Carlos CE, Fonseca Moreira J-C. Increased blood oxidative stress in experimental menopause rat model: the effects of vitamin A low-dose supplementation upon antioxidant status in bilateral ovariectomized rats. Fundam Clin Pharmacol. 2012 Apr;26(2):235-49. doi:
17. Drogan D, Weikert C, Dierkes J, Klipstein-Grobusch K, Buijsse B, Möhlig M, et al. Plasma gamma-glutamyltransferase, cysteinyl-glycine, and oxidized low-density lipoprotein: a pathway associated with myocardial infarction risk? Arterioscler Thromb Vasc Biol. 2010 Oct;30(10):2053-8. doi:

Information about authors:
Kolbasova E.A. – Candidate of Medical Sciences, associate professor of the Chair of Obstetrics and Gynecology, Vitebsk State Order of Peoples’ Friendship Medical University;
Kiseleva N.I. – Doctor of Medical Sciences, professor, head of the Chair of Obstetrics and Gynecology, Vitebsk State Order of Peoples’ Friendship Medical University;
Naumov A.V. – Candidate of Medical Sciences, associate professor of the Chair of Biological Chemistry, Grodno State Medical University.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Obstetrics and Gynecology. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Elena A. Kolbasova.

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