Menu

A+ A A-

Download article

DOI: https://doi.org/10.22263/2312-4156.2021.2.56

Ziamko V.Y.1, Okulich V.K.1, Dzyadzko A.M.2, Kolchanova N.E.1, Goncharova A.I.1, Kornilov A.V.1, Kabanava А.А.1, Averchankava N.A.3
The dependence of microorganisms biofilm formation on the features of the infectious process
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology, Minsk, Republic of Belarus
3Ziegelberg Women’s Hospital, Aschaffenburg, Germany

Vestnik VGMU. 2021;20(2):56-64.

Abstract.
Objectives. To study the dependence of the formation intensity of microbial biofilms by isolates from patients on the severity and localization of the infectious process.
Material and methods. The study conducted from 2016 to 2020 included 894 clinical isolates from 720 patients with infectious pathology of various localization and severety. The formation intensity of microbial biofilms by isolates received from these patients has been studied, depending on the severity and localization of the infectious process.
Results. The property to form biofilms was found out in the overwhelming majority of tested isolates (86.6%). At the same time P. aeruginosa isolates had the maximum biofilm-forming ability, the weight of the microbial biofilm of which was 48.25; 30.1-70.2 mcg/well. The conducted studies have shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated. A direct close relationship was found between the severity of the disease and the mass of microbial biofilm of P. aeruginosа and K. pneumoniae, a weak one – in Acinetobacter spp. (r=0.73, r=0.7 and r=0.35, p<0.05 accordingly).
Conclusions. It can be assumed that intensive formation of biofilms by clinical isolates is an important factor of the aggressiveness and severity of the infectious process. Moreover, the conducted study has shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated.
Key words: biofilm, infections, P. aeruginosа, K. pneumoniae, Acinetobacter spp., S. aureus, Streptococcus spp., E. coli.

References

1. Flemming HC, Wingender J, Szewzyk U, Steinberg P, Rice SA, Kjelleberg S. Biofilms: an emergent form of bacterial life. Nat Rev Microbiol. 2016 Aug;14(9):563-75. doi: http://dx.doi.org/10.1038/nrmicro.2016.94
2. Maurice NM, Bedi B, Sadikot RT. Pseudomonas aeruginosa Biofilms: Host Response and Clinical Implications in Lung Infections. Am J Respir Cell Mol Biol. 2018 Apr;58(4):428-439. doi: http://dx.doi.org/10.1165/rcmb.2017-0321TR
3. Smith S, Waters V, Jahnke N, Ratjen F. Standard versus biofilm antimicrobial susceptibility testing to guide antibiotic therapy in cystic fibrosis. Cochrane Database Syst Rev. 2020 Jun;6(6):CD009528. doi: http://dx.doi.org/10.1002/14651858.CD009528
4. Popat R, Crusz SA, Messina M, Williams P, West SA, Diggle SP. Quorum-sensing and cheating in bacterial biofilms. Proc Biol Sci. 2012 Dec;279(1748):4765-71. doi: http://dx.doi.org/10.1098/rspb.2012.1976
5. Rizzato C, Torres J, Kasamatsu E, Camorlinga-Ponce M, Bravo MM, Canzian F, et al. Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection. Front Microbiol. 2019 Apr;10:846. doi: http://dx.doi.org/10.3389/fmicb.2019.00846
6. Kostakioti M, Hadjifrangiskou M, Hultgren SJ. Bacterial Biofilms: Development, Dispersal, and Therapeutic Strategies in the Dawn of the Postantibiotic Era. Cold Spring Harb Perspect Med. 2013 Apr;3(4):a010306. doi: http://dx.doi.org/10.1101/cshperspect.a010306
7. Pérez-Granda MJ, Latorre MC, Alonso B, Hortal J, Samaniego R, Bouza E, et al. Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study. BMC Infect Dis. 2017 Dec;17(1):746. doi: http://dx.doi.org/10.1186/s12879-017-2856-0
8. Kolomietc ND, Tonko OV, Serookaia TI, Mareiko AM, Litunovskaia LG. Microbiological methods of research of biological material: instruktsiia po primeneniiu № 075-0210: utv M-vom zdravookhraneniia Resp Belarus', 13 marta 2010 g. Minsk,, RB; 2010. 124 р. (In Russ.)
9. Okulich VK, Kabanova AA, Plotnikov FV. Microbial biofilms in clinical microbiology and antibacterial therapy. Vitebsk, RB: VGMU; 2017. 300 р. (In Russ.)
10. Okulich VK, Kolchanova NE, Kornilov AV, Zemko VIu; zaiavitel' i patentoobladatel' Vitebskii gos med un-t. A method for determining the percentage of DNA in the composition of microbial biofilm: pat Resp. Belarus': MKP C 12Q 1/6809, G 01N 21/64. № a 20160477; zaiavl 18.06.18; opubl 02.07.20, Afits biul № 6. Р. 96. (In Russ.)
11. Rebrova OIu. Statistical analysis of medical data. Application Package of Application Programs Statistica. Moscow, RF: Media Sfera; 2006. 312 р. (In Russ.)
12. Lagun LV, Zhavoronok SV. Bacterial biofilms and their role in the development of urinary tract infections. Med Zhurn. 2013;(4):21-7. (In Russ.)
13. Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010 Jun;5(6):917-33. doi: http://dx.doi.org/10.2217/fmb.10.56
14. Plotnikov FV, Okulich VK, Kabanova AA, Shilin VE. The influence of the ability of microorganisms to form a bioflighter on their sensitivity to antibacterial drugs. Immunopatologiia Allergologiia Infektologiia. 2014;(2):52-60. (In Russ.)

Information about authors:
Ziamko V.Y. – Candidate of Medical Sciences, associate professor of the Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-6753-2074;
Okulich V.K. – Candidate of Medical Sciences, associate professor of the Chair of Clinical Microbiology, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405;
Dzyadzko A.M. – Doctor of Medical Sciences, head of the Department of Anesthesiology and Resuscitation, Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology,
ORCID: https://orcid.org/0000-0003-1965-1850;
Kolchanova N.E. – Candidate of Medical Sciences, associate professor of the Chair of Restorative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-3071-1001;
Goncharova A.I. – Candidate of Medical Sciences, associate professor of the Chair of Maxillofacial Surgery & Operative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-8226-6405;
Kornilov A.V. – lecturer of the Chair of Hospital Surgery with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0001-8709-6639;
Kabanava A.A. – Candidate of Medical Sciences, associate professor, head of the Chair of Maxillofacial Surgery & Operative Dentistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
ORCID: https://orcid.org/0000-0002-0121-1139;
Аverchankava N.A. – obstetrician-gynecologist in Ziegelberg Women’s Hospital, Aschaffenburg, Germany.

Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Anesthesiology and Resuscitation with the course of the Faculty for Advanced Training & Retraining. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viktoriya Y. Ziamko.

Поиск по сайту