DOI: https://doi.org/10.22263/2312-4156.2021.2.65
Semenov V.M., Pabiarzhyn V.V., Pashinskaya E.S., Egorov S.K., Gorbachev V.V., Kosova M.S.
The development of test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals using PCR with fluorescence detection and evaluation of their effectiveness
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2021;20(2):65-70.
Abstract.
Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method.
Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment.
Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective.
Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.
Key words: BIRC5 and HER-2/neu, experiment, expression, rats.
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Information about authors:
Semenov V.M. – Doctor of Medical Sciences, professor, head of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Pabiarzhyn V.V. – Candidate of Biological Sciences, associate professor of the Chair of Biology & Pharmaceutical Botany, Vitebsk State Order of Peoples’ Friendship Medical University;
Pashinskaya Е.S. – Candidate of Biological Sciences, associate professor of the Chair of Biology & Pharmaceutical Botany, Vitebsk State Order of Peoples’ Friendship Medical University;
Egorov S.K. – Candidate of Medical Sciences, associate professor of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Gorbachev V.V. – lecturer of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Kosova M.S. – postgraduate of the Chair of Infectious Diseases with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University.
Correspondence address: Republic of Belarus, 210009, Vitebsk, 27 Frunze ave., Vitebsk State Order of Peoples’ Friendship Medical University, Chair of Biology & Pharmaceutical Botany. E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Viachaslau V. Pabiarzhyn.
