Minina E.S.1, Novikova V.I.1, Novikov P.D.1, Babenka A.S.2
The analysis of the association of ADRB2 gene polymorphism and external respiratory function in children with bronchial asthma
1Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
2Belarusian State Medical University, Minsk, Republic of Belarus
Vestnik VGMU. 2021;20(2):71-76.
Objectives. To study the role of the ADRB2 gene polymorphism (rs1042713) in the impaired external respiratory function in children with bronchial asthma (BA).
Material and methods. The study group included 60 children aged 3-17 years with allergic (n=37) and mixed form of BA (n=23). The genotyping of the investigated gene locus was performed by PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism), BA control was exercised using the Asthma Control Test (ACT).
Results. The analysis of allele frequencies showed an association of allele A with normal spirogram parameters in patients with BA (p=0.042). In children under 12 years, there were statistically significant differences in spirogram indices when comparing patients with genotypes AA and GG (p=0.045). An association of allele A with the absence of pathological changes was revealed when evaluating the results of spirometry (p=0.021). There was a moderate positive correlation (r=0.615, p=0.011) between the assessment of nocturnal symptoms (Asthma Control Test) and the genotype of the ADRB2 gene polymorphism in children aged 12 years and older.
Conclusions. An association of the ADRΒ2 gene polymorphism (rs1042713) with the external respiratory function was revealed in an objective study by spirometry and a subjective assessment of this indicator by the patient himself/herself or by his/her parents. This gene locus can be included in the BA target panel in order to determine the likelihood of uncontrolled course development of the disease with impaired external respiratory function and in the future to develop an individual program for dispensary observation of the patient.
Key words: bronchial asthma, ADRΒ2, external respiratory function.
Information about the source of support: the study was carried out within the framework of an intrauniversity scientific startup grant for young scientists for 2020, Vitebsk State Order of Peoples’ Friendship Medical University.
1. Vsemirnaia organizatsiia zdravookhraneniia. Asthma. Available from: https://www.who.int/ru/news-room/fact-sheets/detail/asthma. [Accessed 10th Apr 2021]. (In Russ.)
2. Global initiative for asthma. Global Strategy for Asthma Management and Prevention 2020. Available from: https://ginasthma.org/reports/. [Accessed 10th Apr 2021].
3. Astafeva NG, Shamgunova BA, Kobzev DIu, Mikhailova IE. Associative connections between atopia, HLA complex genes and other genes. Ros Allergol Zhurn. 2019;16(3):5-25. (In Russ.)
4. Minina ES, Novikova VI; M-vo obrazovaniia Resp Belarus', UO Viteb gos ordena Druzhby narodov med un-t. Bronchial asthma in children: Features of treatment and rehabilitation: monografiia. Vitebsk, RB: VGMU; 2017. 274 р. (In Russ.)
5. Saveleva ON, Karunas AS, Fedorova IuIu, Khusnutdinova EK. Pharmacogenetics of bronchial asthma. Med Genetika. 2019;18(4):3-23. (In Russ.)
6. Almomani BA, Al-Eitan LN, Al-Sawalha NA, Samrah SM, Al-Quasmi MN. Association of genetic variants with level of asthma control in the Arab population. J Asthma Allergy. 2019 Jan;12:35-42. doi: http://dx.doi.org/10.2147/JAA.S186252
7. Dai L-M, Wang Z, Zhang Y, Liu L, Fang L, Zhang J. Relationship between the locus 16 genotype of beta 2 adrenergic receptor and the nocturnal asthma phenotype Sichuan. Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Jan;35(1):32-4.
8. Finkelstein Y, Bournissen FG, Hutson JR, Shannon M. Polymorphism of the ADRB2 gene and response to inhaled beta- agonists in children with asthma: a meta-analysis. J Asthma. 2009 Nov;46(9):900-5. doi: http://dx.doi.org/10.3109/02770900903199961
9. Holloway JW, Dunbar PR, Riley G, Sawyer GM, Fitzharris PF, Pearce N, et al. Association of beta2-adrenergic receptor polymorphisms with severe asthma. Clin Exp Allergy. 2000 Aug;30(8):1097-103. doi: http://dx.doi.org/10.1046/j.1365-2222.2000.00929.x
Information about authors:
Minina E.S. – Candidate of Medical Sciences, lecturer of the Chair of Pediatrics, Vitebsk State Order of Peoples’ Friendship Medical University;
Novikova V.I. – Doctor of Medical Sciences, professor, head of the Chair of Pediatrics of the Faculty for Advanced Training and Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Novikov P.D. – Doctor of Medical Sciences, professor of the Chair of Clinical Immunology and Allergology with the course of the Faculty for Advanced Training and Retraining, Vitebsk State Order of Peoples’ Friendship Medical University;
Babenka A.S. – Candidate of Chemical Sciences, associate professor of the Chair of Bioorganic Chemistry, Belarusian State Medical University.