DOI: https://doi.org/10.22263/2312-4156.2023.3.45
A.-M.V. Yerofeyeva
Changes in gait parameters of rats with peripheral neuropathic pain in mesenchymal stem cell transplantation and pharmacological blockade of cannabinoid receptors of type 2
Institute of Physiology of the National Academy of Sciences of Belarus, Minsk, Republic of Belarus
Vestnik VGMU. 2023;22(3):45-54.
Abstract.
Objectives. To evaluate the effect of pharmacological blockade of cannabinoid CB2 receptors on the gait parameters of experimental animals with peripheral neuropathic pain and transplantation of adipose-derived mesenchymal stem cells (ADMSCs).
Material and methods. In 40 male Wistar rats, modelling of peripheral neuropathy (NP) was performed by excising a section of the sciatic nerve of the left hind paw. On the 7th day of the study, the transplantation of ADMSCs (1×106 cells/kg of rat body weight) into the area of sciatic nerve injury was done without any additional interventions and after administration of the CB2 receptor antagonist AM630 (100 μg/kg of rat body weight), as well as the transplantation of ADMSCs pretreated with AM630. For 90 days, the gait parameters of experimental animals were being studied using the CatWalk XT detailed gait analysis system (Noldus, Netherlands).
Results. Transplantation of ADMSCs into the area of rat’s sciatic nerve transection accelerated the recovery of the dynamic gait parameters and abolished the development of disturbances in the static gait parameters. The administration of AM630 into the area of sciatic nerve transection before the transplantation of ADMSCs contributed to the development of gait disturbances (stand duration of the injured hind paw, duty cycle of the injured hind paw, paw print area, maximum and mean paw print intensity, functional sciatic index) and was accompanied by a pronounced lesion of distal segment of the sciatic nerve with an increase in the level of proliferation of Schwann cells. Transplantation of ADMSCs pretreated with AM630 had no significant effect on the rate of recovery of static and dynamic gait parameters, but led to a later recovery of the functional sciatic index.
Conclusions. Persistence of gait disturbances in experimental animals after CB2 receptors blockade both on MSC’s membranes and in the area of peripheral nerve damage indicates suppression of the antinociceptive and reparative action of MSCs. CB2 receptors in the soft tissues of the area of nerve damage are more involved and influence the implementation of these effects of stem cells.
Keywords: mesenchymal stem cells, neuropathic pain, sciatic nerve, cannabinoid receptors, gait analysis, catwalk.
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Information about authors:
A.-M.V. Yerofeyeva – postgraduate, associate research officer of the biological simulation laboratory, Institute of Physiology of the National Academy of Sciences of Belarus, https://orcid.org/0000-0002-9407-9295,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Anna-Maria V. Yerofeyeva.
