DOI: https://doi.org/10.22263/2312-4156.2023.6.18
K.V. Pyko, S.S. Osochuk
The effect of regular physical activity on zeta potential value and protein-carbohydrate composition of the erythrocyte membrane in humans
Vitebsk State Order of Peoples’ Friendship Medical University, Vitebsk, Republic of Belarus
Vestnik VGMU. 2023;22(6):18-24.
Abstract.
Objectives. To study the effect of regular physical activity on protein-carbohydrate composition of the erythrocyte membrane and zeta potential value.
Material and methods. Blood samples of 36 men aged 28.5±5 years with regular normalized physical activity and 48 men aged 21.5±2 years without regular physical activity served as the object of the study. The value of zeta potential (ζ-potential), the content of total sialic acids, total protein, N-acetylneuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc), band 3 protein, glycophorin A (GPA), as well as the concentration of neuraminidase in blood plasma were determined in erythrocytes of all subjects.
Results. In the composition of erythrocyte membranes of individuals with regular physical activity a decrease in the content of total protein, total sialic acids, glycophorin A, NeuAc, NeuGc was revealed, but the specific charge per 1 ng of NeuAc and NeuGc increased, and per 1 ng of GPA was decreased. Protein-normalized sialic acid and GPA content was decreased, that of band 3 protein – increased.
Conclusions. Regular exercise significantly alters the composition of erythrocyte membranes, probably, making it more functional by increasing sialic acid-normalized ζ-potential and increasing specific band 3 protein content.
Keywords: sialic acids, zeta potential, erythrocyte, glycophorin A, band 3 protein, N-acetylneuraminic acid.
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Information about authors:
K.V. Pyko – postgraduate of the Chair of General & Clinical Biochemistry with the course of the Faculty for Advanced Training & Retraining, Vitebsk State Order of Peoples’ Friendship Medical University,
e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра. – Kirill V. Pyko;
S.S. Osochuk – Doctor of Medical Sciences, professor, head of the research laboratory, Vitebsk State Order of Peoples’ Friendship Medical University.
